1lci

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(New page: 200px<br /><applet load="1lci" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lci, resolution 2.0&Aring;" /> '''FIREFLY LUCIFERASE'''...)
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[[Image:1lci.jpg|left|200px]]<br /><applet load="1lci" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lci, resolution 2.0&Aring;" />
 
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'''FIREFLY LUCIFERASE'''<br />
 
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==Overview==
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==FIREFLY LUCIFERASE==
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BACKGROUND. Firefly luciferase is a 62 kDa protein that catalyzes the, production of light. In the presence of MgATP and molecular oxygen, the, enzyme oxidizes its substrate, firefly luciferin, emitting yellow-green, light. The reaction proceeds through activation of the substrate to form, an adenylate intermediate. Firefly luciferase shows extensive sequence, homology with a number of enzymes that utilize ATP in adenylation, reactions. RESULTS. We have determined the crystal structure of firefly, luciferase at 2.0 A resolution. The protein is folded into two compact, domains. The large N-terminal domain consists of a beta-barrel and two, beta-sheets. The sheets are flanked by alpha-helices to form an, alphabetaalphabetaalpha five-layered structure. The C-terminal portion of, the molecule forms a distinct domain, which is separated from the, N-terminal domain by a wide cleft. CONCLUSIONS. Firefly luciferase is the, first member of a superfamily of homologous enzymes, which includes, acyl-coenzyme A ligases and peptide synthetases, to have its structure, characterized. The residues conserved within the superfamily are located, on the surfaces of the two domains on either side of the cleft, but are, too far apart to interact simultaneously with the substrates. This, suggests that the two domains will close in the course of the reaction., Firefly luciferase has a novel structural framework for catalyzing, adenylate-forming reactions.
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<StructureSection load='1lci' size='340' side='right'caption='[[1lci]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lci]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LCI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lci OCA], [https://pdbe.org/1lci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lci RCSB], [https://www.ebi.ac.uk/pdbsum/1lci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lci ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY] Produces green light with a wavelength of 562 nm.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lc/1lci_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lci ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LCI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Active as [http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LCI OCA].
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*[[Luciferase 3D structures|Luciferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes., Conti E, Franks NP, Brick P, Structure. 1996 Mar 15;4(3):287-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8805533 8805533]
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[[Category: Large Structures]]
[[Category: Photinus pyralis]]
[[Category: Photinus pyralis]]
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[[Category: Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)]]
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[[Category: Brick P]]
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[[Category: Single protein]]
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[[Category: Conti E]]
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[[Category: Brick, P.]]
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[[Category: Franks NP]]
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[[Category: Conti, E.]]
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[[Category: Franks, N.P.]]
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[[Category: luminescence]]
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[[Category: monooxygenase]]
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[[Category: oxidoreductase]]
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[[Category: photoprotein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:28:39 2007''
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Current revision

FIREFLY LUCIFERASE

PDB ID 1lci

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