1lcu

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(New page: 200px<br /><applet load="1lcu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lcu, resolution 3.5&Aring;" /> '''Polylysine Induces an...)
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[[Image:1lcu.jpg|left|200px]]<br /><applet load="1lcu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lcu, resolution 3.5&Aring;" />
 
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'''Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution'''<br />
 
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==Overview==
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==Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution==
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An antiparallel actin dimer has been proposed to be an intermediate, species during actin filament nucleation. We now show that latrunculin A, a marine natural product that inhibits actin polymerization, arrests, polylysine-induced nucleation at the level of an antiparallel dimer, resulting in its accumulation. These dimers, when composed of, pyrene-labeled actin subunits, give rise to a fluorescent excimer, permitting detection during polymerization in vitro. We report the, crystallographic structure of the polylysine-actin-latrunculin A complex, at 3.5-A resolution. The non-crystallographic contact is consistent with a, dimeric structure and confirms the antiparallel orientation of its, subunits. The crystallographic contacts reveal that the mobile DNase I, binding loop of one subunit of a symmetry-related antiparallel actin dimer, is partially stabilized in the interface between the two subunits of a, second antiparallel dimer. These results provide a potential explanation, for the paradoxical nucleation of actin filaments that have exclusively, parallel subunits by a dimer containing antiparallel subunits.
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<StructureSection load='1lcu' size='340' side='right'caption='[[1lcu]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lcu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LCU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LAR:LATRUNCULIN+A'>LAR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lcu OCA], [https://pdbe.org/1lcu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lcu RCSB], [https://www.ebi.ac.uk/pdbsum/1lcu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lcu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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An antiparallel actin dimer has been proposed to be an intermediate species during actin filament nucleation. We now show that latrunculin A, a marine natural product that inhibits actin polymerization, arrests polylysine-induced nucleation at the level of an antiparallel dimer, resulting in its accumulation. These dimers, when composed of pyrene-labeled actin subunits, give rise to a fluorescent excimer, permitting detection during polymerization in vitro. We report the crystallographic structure of the polylysine-actin-latrunculin A complex at 3.5-A resolution. The non-crystallographic contact is consistent with a dimeric structure and confirms the antiparallel orientation of its subunits. The crystallographic contacts reveal that the mobile DNase I binding loop of one subunit of a symmetry-related antiparallel actin dimer is partially stabilized in the interface between the two subunits of a second antiparallel dimer. These results provide a potential explanation for the paradoxical nucleation of actin filaments that have exclusively parallel subunits by a dimer containing antiparallel subunits.
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==About this Structure==
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Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution.,Bubb MR, Govindasamy L, Yarmola EG, Vorobiev SM, Almo SC, Somasundaram T, Chapman MS, Agbandje-McKenna M, McKenna R J Biol Chem. 2002 Jun 7;277(23):20999-1006. Epub 2002 Apr 3. PMID:11932258<ref>PMID:11932258</ref>
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1LCU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with CA, CL, ATP and LAR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LCU OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution., Bubb MR, Govindasamy L, Yarmola EG, Vorobiev SM, Almo SC, Somasundaram T, Chapman MS, Agbandje-McKenna M, McKenna R, J Biol Chem. 2002 Jun 7;277(23):20999-1006. Epub 2002 Apr 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11932258 11932258]
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</div>
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[[Category: Oryctolagus cuniculus]]
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<div class="pdbe-citations 1lcu" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Agbandje-Mckenna, M.]]
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[[Category: Almo, S.C.]]
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[[Category: Bubb, M.R.]]
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[[Category: Chapman, M.S.]]
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[[Category: Govindasamy, L.]]
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[[Category: Mckenna, R.]]
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[[Category: Somasundaram, T.]]
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[[Category: Vorobiev, S.M.]]
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[[Category: Yarmola, E.G.]]
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[[Category: ATP]]
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[[Category: CA]]
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[[Category: CL]]
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[[Category: LAR]]
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[[Category: muscle protein]]
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[[Category: structural protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:29:02 2007''
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==See Also==
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*[[Actin 3D structures|Actin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Oryctolagus cuniculus]]
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[[Category: Agbandje-Mckenna M]]
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[[Category: Almo SC]]
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[[Category: Bubb MR]]
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[[Category: Chapman MS]]
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[[Category: Govindasamy L]]
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[[Category: Mckenna R]]
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[[Category: Somasundaram T]]
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[[Category: Vorobiev SM]]
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[[Category: Yarmola EG]]

Current revision

Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution

PDB ID 1lcu

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