2eyu

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{{Seed}}
 
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[[Image:2eyu.png|left|200px]]
 
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==The Crystal Structure of the C-terminal Domain of Aquifex aeolicus PilT==
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The line below this paragraph, containing "STRUCTURE_2eyu", creates the "Structure Box" on the page.
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<StructureSection load='2eyu' size='340' side='right'caption='[[2eyu]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2eyu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EYU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2eyu| PDB=2eyu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eyu OCA], [https://pdbe.org/2eyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eyu RCSB], [https://www.ebi.ac.uk/pdbsum/2eyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eyu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O66950_AQUAE O66950_AQUAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/2eyu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eyu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PilT is a hexameric ATPase required for bacterial type IV pilus retraction and surface motility. Crystal structures of ADP- and ATP-bound Aquifex aeolicus PilT at 2.8 and 3.2 A resolution show N-terminal PAS-like and C-terminal RecA-like ATPase domains followed by a set of short C-terminal helices. The hexamer is formed by extensive polar subunit interactions between the ATPase core of one monomer and the N-terminal domain of the next. An additional structure captures a nonsymmetric PilT hexamer in which approach of invariant arginines from two subunits to the bound nucleotide forms an enzymatically competent active site. A panel of pilT mutations highlights the importance of the arginines, the PAS-like domain, the polar subunit interface, and the C-terminal helices for retraction. We present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this hexameric motor. Our conclusions apply to the entire type II/IV secretion ATPase family.
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===The Crystal Structure of the C-terminal Domain of Aquifex aeolicus PilT===
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Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility.,Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT Structure. 2007 Mar;15(3):363-76. PMID:17355871<ref>PMID:17355871</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17355871}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2eyu" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17355871 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17355871}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2EYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EYU OCA].
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==Reference==
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Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility., Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT, Structure. 2007 Mar;15(3):363-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17355871 17355871]
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The pilus-retraction protein PilT: ultrastructure of the biological assembly., Forest KT, Satyshur KA, Worzalla GA, Hansen JK, Herdendorf TJ, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):978-82. Epub 2004, Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15103158 15103158]
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[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aukema, K G.]]
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[[Category: Aukema KG]]
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[[Category: Forest, K T.]]
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[[Category: Forest KT]]
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[[Category: Heiniger, E K.]]
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[[Category: Heiniger EK]]
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[[Category: Meyer, L S.]]
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[[Category: Meyer LS]]
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[[Category: Satyshur, K A.]]
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[[Category: Satyshur KA]]
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[[Category: Worzalla, G A.]]
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[[Category: Worzalla GA]]
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[[Category: C-terminal domain pilt]]
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[[Category: Pilus retraction motor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:23:55 2008''
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Current revision

The Crystal Structure of the C-terminal Domain of Aquifex aeolicus PilT

PDB ID 2eyu

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