1nhc

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{{Seed}}
 
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[[Image:1nhc.png|left|200px]]
 
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==Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger==
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The line below this paragraph, containing "STRUCTURE_1nhc", creates the "Structure Box" on the page.
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<StructureSection load='1nhc' size='340' side='right'caption='[[1nhc]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nhc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NHC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1nhc| PDB=1nhc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nhc OCA], [https://pdbe.org/1nhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nhc RCSB], [https://www.ebi.ac.uk/pdbsum/1nhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nhc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGLR1_ASPNG PGLR1_ASPNG] Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nh/1nhc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nhc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Endopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred.
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===Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger===
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Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger.,van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112<ref>PMID:14623112</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14623112}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1nhc" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14623112 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14623112}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1NHC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHC OCA].
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==Reference==
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Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14623112 14623112]
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[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Polygalacturonase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Benen JA]]
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[[Category: Benen, J A.]]
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[[Category: Dijkstra BW]]
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[[Category: Dijkstra, B W.]]
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[[Category: Snijder HJ]]
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[[Category: Pouderoyen, G van.]]
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[[Category: Van Pouderoyen G]]
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[[Category: Snijder, H J.]]
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[[Category: Beta-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:49:42 2008''
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Current revision

Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger

PDB ID 1nhc

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