2hrt

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{{Seed}}
 
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[[Image:2hrt.png|left|200px]]
 
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==Asymmetric structure of trimeric AcrB from Escherichia coli==
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The line below this paragraph, containing "STRUCTURE_2hrt", creates the "Structure Box" on the page.
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<StructureSection load='2hrt' size='340' side='right'caption='[[2hrt]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2hrt]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HRT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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{{STRUCTURE_2hrt| PDB=2hrt | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hrt OCA], [https://pdbe.org/2hrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hrt RCSB], [https://www.ebi.ac.uk/pdbsum/2hrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hrt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACRB_ECOLI ACRB_ECOLI] AcrAB is a drug efflux protein with a broad substrate specificity.<ref>PMID:16915237</ref> <ref>PMID:16946072</ref> <ref>PMID:17194213</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/2hrt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hrt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The AcrA/AcrB/TolC complex spans the inner and outer membranes of Escherichia coli and serves as its major drug-resistance pump. Driven by the proton motive force, it mediates the efflux of bile salts, detergents, organic solvents, and many structurally unrelated antibiotics. Here, we report a crystallographic structure of trimeric AcrB determined at 2.9 and 3.0 angstrom resolution in space groups that allow asymmetry of the monomers. This structure reveals three different monomer conformations representing consecutive states in a transport cycle. The structural data imply an alternating access mechanism and a novel peristaltic mode of drug transport by this type of transporter.
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===Asymmetric structure of trimeric AcrB from Escherichia coli===
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Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism.,Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos KM Science. 2006 Sep 1;313(5791):1295-8. PMID:16946072<ref>PMID:16946072</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16946072}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2hrt" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16946072 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16946072}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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2HRT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HRT OCA].
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[[Category: Large Structures]]
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[[Category: Diederichs K]]
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==Reference==
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[[Category: Eicher T]]
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Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism., Seeger MA, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos KM, Science. 2006 Sep 1;313(5791):1295-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16946072 16946072]
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[[Category: Pos KM]]
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[[Category: Escherichia coli]]
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[[Category: Schiefner A]]
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[[Category: Single protein]]
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[[Category: Seeger MA]]
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[[Category: Diederichs, K.]]
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[[Category: Verrey F]]
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[[Category: Eicher, T.]]
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[[Category: Pos, K M.]]
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[[Category: Schiefner, A.]]
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[[Category: Seeger, M A.]]
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[[Category: Verrey, F.]]
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[[Category: Acra]]
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[[Category: Acrb]]
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[[Category: Antibiotic resistance]]
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[[Category: Membrane protein]]
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[[Category: Multidrug efflux pump]]
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[[Category: Proton-coupled exchanger]]
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[[Category: Rnd]]
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[[Category: Tolc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:51:12 2008''
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Current revision

Asymmetric structure of trimeric AcrB from Escherichia coli

PDB ID 2hrt

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