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1lji

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(New page: 200px<br /><applet load="1lji" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lji, resolution 2.00&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1lji.gif|left|200px]]<br /><applet load="1lji" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lji, resolution 2.00&Aring;" />
 
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'''CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE 10% SORBITOL'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE 10% SORBITOL==
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In pursuance of a long-range programme on the hydration, mobility and, action of proteins, the structural basis of the stabilizing effect of, sugars and polyols is being investigated. With two crystallographically, independent molecules with slightly different packing environments in the, crystal, monoclinic lysozyme constitutes an ideal system for exploring the, problem. The differences in the structure and hydration of the two, molecules provide a framework for examining the changes caused by, stabilizing additives. Monoclinic crystals were grown under native, conditions and also in the presence of 10% sucrose, 15% trehalose, 10%, trehalose, 10% sorbitol and 5% glycerol. The crystal structures were, refined at resolutions ranging from 1.8 to 2.1 A. The average B values, and hence the mobility of the structure, are lower in the presence of, additives than in the native crystals. However, a comparison of the, structures indicates that the effect of the additives on the structure and, the hydration shell around the protein molecule is considerably less than, that caused by differences in packing. It is also less than that caused by, the replacement of NaNO(3) by NaCl as the precipitant in the, crystallization experiments. This result is not in conformity with the, commonly held belief that additives exert their stabilizing effect through, the reorganization of the hydration shell, at least as far as the ordered, water molecules are concerned.
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<StructureSection load='1lji' size='340' side='right'caption='[[1lji]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lji]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LJI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lji OCA], [https://pdbe.org/1lji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lji RCSB], [https://www.ebi.ac.uk/pdbsum/1lji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lji ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lj/1lji_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lji ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LJI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with NO3 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LJI OCA].
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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==Reference==
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<references/>
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Effect of stabilizing additives on the structure and hydration of proteins: a study involving monoclinic lysozyme., Saraswathi NT, Sankaranarayanan R, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1162-7. Epub 2002, Jun 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12077436 12077436]
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__TOC__
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Sankaranarayanan R]]
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[[Category: Sankaranarayanan, R.]]
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[[Category: Saraswathi NT]]
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[[Category: Saraswathi, N.T.]]
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[[Category: Vijayan M]]
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[[Category: Vijayan, M.]]
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[[Category: NO3]]
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[[Category: hydration of proteins]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:36:09 2007''
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CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE 10% SORBITOL

PDB ID 1lji

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