2frp

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{{Seed}}
 
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[[Image:2frp.png|left|200px]]
 
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==Bacteriophage HK97 Expansion Intermediate IV==
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The line below this paragraph, containing "STRUCTURE_2frp", creates the "Structure Box" on the page.
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<SX load='2frp' size='340' side='right' viewer='molstar' caption='[[2frp]], [[Resolution|resolution]] 7.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2frp]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FRP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2frp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2frp OCA], [https://pdbe.org/2frp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2frp RCSB], [https://www.ebi.ac.uk/pdbsum/2frp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2frp ProSAT]</span></td></tr>
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{{STRUCTURE_2frp| PDB=2frp | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/2frp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2frp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 A radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.
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===Bacteriophage HK97 Expansion Intermediate IV===
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Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM.,Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE Structure. 2006 Nov;14(11):1655-65. PMID:17098191<ref>PMID:17098191</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17098191}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2frp" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17098191 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17098191}}
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__TOC__
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</SX>
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==About this Structure==
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[[Category: Escherichia virus HK97]]
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2FRP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk97 Enterobacteria phage hk97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FRP OCA].
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[[Category: Large Structures]]
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[[Category: Cheng N]]
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==Reference==
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[[Category: Conway JF]]
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Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098191 17098191]
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[[Category: Duda RL]]
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[[Category: Enterobacteria phage hk97]]
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[[Category: Firek BA]]
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[[Category: Single protein]]
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[[Category: Gan L]]
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[[Category: Cheng, N.]]
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[[Category: Hendrix RW]]
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[[Category: Conway, J F.]]
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[[Category: Johnson JE]]
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[[Category: Duda, R L.]]
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[[Category: Lander G]]
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[[Category: Firek, B A.]]
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[[Category: Liljas L]]
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[[Category: Gan, L.]]
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[[Category: Speir JA]]
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[[Category: Hendrix, R W.]]
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[[Category: Johnson, J E.]]
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[[Category: Lander, G.]]
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[[Category: Liljas, L.]]
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[[Category: Speir, J A.]]
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[[Category: Bacteriophage]]
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[[Category: Capsid protein]]
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[[Category: Expansion intermediate]]
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[[Category: Hk97]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:13:11 2008''
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Current revision

Bacteriophage HK97 Expansion Intermediate IV

2frp, resolution 7.50Å

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OCA

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