1llo

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(New page: 200px<br /><applet load="1llo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1llo, resolution 1.85&Aring;" /> '''HEVAMINE A (A PLANT ...)
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[[Image:1llo.gif|left|200px]]<br /><applet load="1llo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1llo, resolution 1.85&Aring;" />
 
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'''HEVAMINE A (A PLANT ENDOCHITINASE/LYSOZYME) COMPLEXED WITH ALLOSAMIDIN'''<br />
 
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==Overview==
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==HEVAMINE A (A PLANT ENDOCHITINASE/LYSOZYME) COMPLEXED WITH ALLOSAMIDIN==
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The plant enzyme hevamine has both chitinase and lysozyme activity. HPLC, analysis of the products of the hydrolysis of chitopentaose shows that, hevamine acts with retention of the configuration, despite the absence of, a nucleophilic or stabilizing carboxylate. To analyze the stabilization of, a putative oxocarbonium ion intermediate, the X-ray structure of hevamine, complexed with the inhibitor allosamidin was determined at 1.85 A, resolution. This structure supports the role of Glu127 as a proton donor., The allosamizoline group binds in the center of the active site, mimicking, a reaction intermediate in which a positive charge at C1 is stabilized, intramolecularly by the carbonyl oxygen of the N-acetyl group at C2.
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<StructureSection load='1llo' size='340' side='right'caption='[[1llo]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1llo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LLO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMI:ALLOSAMIZOLINE'>AMI</scene>, <scene name='pdbligand=NAA:N-ACETYL-D-ALLOSAMINE'>NAA</scene>, <scene name='pdbligand=PRD_000468:Allosamidin'>PRD_000468</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1llo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1llo OCA], [https://pdbe.org/1llo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1llo RCSB], [https://www.ebi.ac.uk/pdbsum/1llo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1llo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHLY_HEVBR CHLY_HEVBR] Bifunctional enzyme with lysozyme / chitinase activity. May have a role in plugging the latex vessel and cessation of latex flow.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ll/1llo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1llo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The plant enzyme hevamine has both chitinase and lysozyme activity. HPLC analysis of the products of the hydrolysis of chitopentaose shows that hevamine acts with retention of the configuration, despite the absence of a nucleophilic or stabilizing carboxylate. To analyze the stabilization of a putative oxocarbonium ion intermediate, the X-ray structure of hevamine complexed with the inhibitor allosamidin was determined at 1.85 A resolution. This structure supports the role of Glu127 as a proton donor. The allosamizoline group binds in the center of the active site, mimicking a reaction intermediate in which a positive charge at C1 is stabilized intramolecularly by the carbonyl oxygen of the N-acetyl group at C2.
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==About this Structure==
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Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: evidence for substrate assisted catalysis.,Terwisscha van Scheltinga AC, Armand S, Kalk KH, Isogai A, Henrissat B, Dijkstra BW Biochemistry. 1995 Dec 5;34(48):15619-23. PMID:7495789<ref>PMID:7495789</ref>
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1LLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis] with AMI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LLO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: evidence for substrate assisted catalysis., Terwisscha van Scheltinga AC, Armand S, Kalk KH, Isogai A, Henrissat B, Dijkstra BW, Biochemistry. 1995 Dec 5;34(48):15619-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7495789 7495789]
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</div>
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<div class="pdbe-citations 1llo" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Hevea brasiliensis]]
[[Category: Hevea brasiliensis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Armand, S.]]
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[[Category: Armand S]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Dijkstra BW]]
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[[Category: Henrissat, B.]]
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[[Category: Henrissat B]]
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[[Category: Isogai, A.]]
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[[Category: Isogai A]]
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[[Category: Kalk, K.H.]]
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[[Category: Kalk KH]]
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[[Category: Scheltinga, A.C.Terwisscha.Van.]]
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[[Category: Terwisscha Van Scheltinga AC]]
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[[Category: AMI]]
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[[Category: chitinase]]
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[[Category: lysozyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:39:32 2007''
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Current revision

HEVAMINE A (A PLANT ENDOCHITINASE/LYSOZYME) COMPLEXED WITH ALLOSAMIDIN

PDB ID 1llo

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