2orc

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{{Seed}}
 
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[[Image:2orc.png|left|200px]]
 
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==CRO REPRESSOR INSERTION MUTANT K56-[DGEVK], NMR, 32 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_2orc", creates the "Structure Box" on the page.
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<StructureSection load='2orc' size='340' side='right'caption='[[2orc]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2orc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ORC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2orc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2orc OCA], [https://pdbe.org/2orc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2orc RCSB], [https://www.ebi.ac.uk/pdbsum/2orc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2orc ProSAT]</span></td></tr>
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{{STRUCTURE_2orc| PDB=2orc | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RCRO_LAMBD RCRO_LAMBD] Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/2orc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2orc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of a monomeric variant of the lambda Cro repressor has been determined by multidimensional NMR. Cro K56[DGEVK] differs from wild-type Cro by the insertion of five amino acids at the center of the dimer interface. 1H and 15N resonances for 70 of the 71 residues have been assigned. Thirty-two structures were calculated by hybrid distance geometry/simulated annealing methods using 463 NOE-distance restraints, 26 hydrogen-bond, and 39 dihedral-angle restraints. The root-mean-square deviation (RMSD) from the average structure for atoms in residues 3-60 is 1.03 +/- 0.44 A for the peptide backbone and 1.6 +/- 0.73 A for all nonhydrogen atoms. The overall structure conforms very well to the original design. Although the five inserted residues form a beta hairpin as expected, this engineered turn as well as other turns in the structure are not well defined by the NMR data. Dynamics studies of backbone amides reveal T1/T2 ratios of residues in the alpha2-alpha3, beta2-beta3, and engineered turn that are reflective of chemical exchange or internal motion. The solution structure and dynamics are discussed in light of the conformational variation that has been observed in other Cro structures, and the importance of flexibility in DNA recognition.
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===CRO REPRESSOR INSERTION MUTANT K56-[DGEVK], NMR, 32 STRUCTURES===
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Solution structure and dynamics of a designed monomeric variant of the lambda Cro repressor.,Mossing MC Protein Sci. 1998 Apr;7(4):983-93. PMID:9568905<ref>PMID:9568905</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_9568905}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2orc" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 9568905 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9568905}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus Lambda]]
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2ORC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORC OCA].
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[[Category: Large Structures]]
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[[Category: Mossing MC]]
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==Reference==
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Solution structure and dynamics of a designed monomeric variant of the lambda Cro repressor., Mossing MC, Protein Sci. 1998 Apr;7(4):983-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9568905 9568905]
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[[Category: Enterobacteria phage lambda]]
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[[Category: Single protein]]
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[[Category: Mossing, M C.]]
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[[Category: Gene regulating protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:37:42 2008''
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Current revision

CRO REPRESSOR INSERTION MUTANT K56-[DGEVK], NMR, 32 STRUCTURES

PDB ID 2orc

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