1u2w

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{{Seed}}
 
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[[Image:1u2w.png|left|200px]]
 
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==Crystal Structure of the Staphylococcus aureus pI258 CadC==
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The line below this paragraph, containing "STRUCTURE_1u2w", creates the "Structure Box" on the page.
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<StructureSection load='1u2w' size='340' side='right'caption='[[1u2w]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1u2w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U2W FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1u2w| PDB=1u2w | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u2w OCA], [https://pdbe.org/1u2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u2w RCSB], [https://www.ebi.ac.uk/pdbsum/1u2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u2w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CADC_STAAU CADC_STAAU] Metal-binding repressor for the cad operon. Involved in resistance to heavy metals, such as cadmium, bismuth, zinc or lead. Binds 2 metal ions per subunit. Metal binding to the N-terminal regulatory site causes the repressor to dissociate from the DNA.<ref>PMID:7543476</ref> <ref>PMID:11278706</ref> <ref>PMID:12176999</ref> <ref>PMID:11941514</ref> <ref>PMID:19286656</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u2/1u2w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u2w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Staphylococcus aureus plasmid pI258 cadCA operon encodes a P-type ATPase, CadA, that confers resistance to the heavy metals Cd(II), Zn(II), and Pb(II). Expression of this heavy-metal efflux pump is regulated by CadC, a homodimeric repressor that dissociates from the cad operator/promoter upon binding of Cd(II), Pb(II), or Zn(II). CadC is a member of the ArsR/SmtB family of metalloregulatory proteins. Here we report the X-ray crystal structure of CadC at 1.9 angstroms resolution. The dimensions of the protein dimer are approximately 30 angstroms by 40 angstroms by 70 angstroms. Each monomer contains six alpha-helices and a three-stranded beta-sheet. Helices 4 and 5 form a classic helix-turn-helix motif that is the putative DNA binding region. The alpha1 helix of one monomer crosses the dimer to approach the alpha4 helix of the other monomer, consistent with the previous proposal that these two regulatory metal binding sites for the inducer cadmium or lead are each formed by Cys-7 and Cys-11 from the N terminus of one monomer and Cys-58 and Cys-60 of the other monomer. Two nonregulatory metal binding sites containing zinc are formed between the two antiparallel alpha6 helices at the dimerization interface. This is the first reported three-dimensional structure of a member of the ArsR/SmtB family with regulatory metal binding sites at the DNA binding domain and the first structure of a transcription repressor that responds to the heavy metals Cd(II) and Pb(II).
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===Crystal Structure of the Staphylococcus aureus pI258 CadC===
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Crystal structure of the Staphylococcus aureus pI258 CadC Cd(II)/Pb(II)/Zn(II)-responsive repressor.,Ye J, Kandegedara A, Martin P, Rosen BP J Bacteriol. 2005 Jun;187(12):4214-21. PMID:15937183<ref>PMID:15937183</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15937183}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1u2w" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15937183 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15937183}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1U2W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U2W OCA].
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==Reference==
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Crystal structure of the Staphylococcus aureus pI258 CadC Cd(II)/Pb(II)/Zn(II)-responsive repressor., Ye J, Kandegedara A, Martin P, Rosen BP, J Bacteriol. 2005 Jun;187(12):4214-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15937183 15937183]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Kandegedara, A.]]
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[[Category: Kandegedara A]]
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[[Category: Martin, P.]]
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[[Category: Martin P]]
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[[Category: Rosen, B P.]]
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[[Category: Rosen BP]]
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[[Category: Ye, J.]]
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[[Category: Ye J]]
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[[Category: Arsr/smtb family]]
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[[Category: Cadmium]]
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[[Category: Lead]]
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[[Category: Repressor]]
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[[Category: Soft metal ion resistance]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:40:54 2008''
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Current revision

Crystal Structure of the Staphylococcus aureus pI258 CadC

PDB ID 1u2w

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