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2v5m
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:2v5m.png|left|200px]] | ||
| - | < | + | ==Structural basis for Dscam isoform specificity== |
| - | + | <StructureSection load='2v5m' size='340' side='right'caption='[[2v5m]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2v5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V5M FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |
| - | -- | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5m OCA], [https://pdbe.org/2v5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v5m RCSB], [https://www.ebi.ac.uk/pdbsum/2v5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5m ProSAT]</span></td></tr> |
| - | + | </table> | |
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v5/2v5m_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v5m ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Dscam gene gives rise to thousands of diverse cell surface receptors thought to provide homophilic and heterophilic recognition specificity for neuronal wiring and immune responses. Mutually exclusive splicing allows for the generation of sequence variability in three immunoglobulin ecto-domains, D2, D3 and D7. We report X-ray structures of the amino-terminal four immunoglobulin domains (D1-D4) of two distinct Dscam isoforms. The structures reveal a horseshoe configuration, with variable residues of D2 and D3 constituting two independent surface epitopes on either side of the receptor. Both isoforms engage in homo-dimerization coupling variable domain D2 with D2, and D3 with D3. These interactions involve symmetric, antiparallel pairing of identical peptide segments from epitope I that are unique to each isoform. Structure-guided mutagenesis and swapping of peptide segments confirm that epitope I, but not epitope II, confers homophilic binding specificity of full-length Dscam receptors. Phylogenetic analysis shows strong selection of matching peptide sequences only for epitope I. We propose that peptide complementarity of variable residues in epitope I of Dscam is essential for homophilic binding specificity. | ||
| - | + | Structural basis of Dscam isoform specificity.,Meijers R, Puettmann-Holgado R, Skiniotis G, Liu JH, Walz T, Wang JH, Schmucker D Nature. 2007 Sep 27;449(7161):487-91. Epub 2007 Aug 26. PMID:17721508<ref>PMID:17721508</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2v5m" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | == | + | [[Category: Drome]] |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Liu, J H]] | |
| - | == | + | [[Category: Meijers, R]] |
| - | + | [[Category: Puettmann-Holgado, R]] | |
| - | [[Category: | + | [[Category: Schmucker, D]] |
| - | [[Category: | + | [[Category: Skiniotis, G]] |
| - | [[Category: Liu, J H | + | [[Category: Walz, T]] |
| - | [[Category: Meijers, R | + | [[Category: Wang, J H]] |
| - | [[Category: Puettmann-Holgado, R | + | |
| - | [[Category: Schmucker, D | + | |
| - | [[Category: Skiniotis, G | + | |
| - | [[Category: Walz, T | + | |
| - | [[Category: Wang, J H | + | |
[[Category: Cell adhesion]] | [[Category: Cell adhesion]] | ||
[[Category: Developmental protein]] | [[Category: Developmental protein]] | ||
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[[Category: Membrane]] | [[Category: Membrane]] | ||
[[Category: Neurobiology spl]] | [[Category: Neurobiology spl]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:42:07 2008'' | ||
Current revision
Structural basis for Dscam isoform specificity
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