1uvk

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{{Seed}}
 
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[[Image:1uvk.png|left|200px]]
 
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==The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 dead-end complex==
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The line below this paragraph, containing "STRUCTURE_1uvk", creates the "Structure Box" on the page.
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<StructureSection load='1uvk' size='340' side='right'caption='[[1uvk]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uvk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UVK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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{{STRUCTURE_1uvk| PDB=1uvk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uvk OCA], [https://pdbe.org/1uvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uvk RCSB], [https://www.ebi.ac.uk/pdbsum/1uvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uvk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RDRP_BPPH6 RDRP_BPPH6]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+.
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===THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX===
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The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase.,Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM Structure. 2004 Feb;12(2):307-16. PMID:14962391<ref>PMID:14962391</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1uvk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_14962391}}, adds the Publication Abstract to the page
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 14962391 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14962391}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1UVK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVK OCA].
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[[Category: Pseudomonas virus phi6]]
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[[Category: Bamford D]]
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==Reference==
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[[Category: Butcher S]]
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The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase., Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM, Structure. 2004 Feb;12(2):307-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962391 14962391]
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[[Category: Grimes JM]]
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[[Category: Protein complex]]
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[[Category: Makeyev EV]]
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[[Category: Pseudomonas phage phi6]]
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[[Category: Salgado PS]]
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[[Category: Bamford, D.]]
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[[Category: Stuart DI]]
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[[Category: Butcher, S.]]
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[[Category: Grimes, J M.]]
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[[Category: Makeyev, E V.]]
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[[Category: Salgado, P S.]]
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[[Category: Stuart, D I.]]
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[[Category: Oligonucleotide]]
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[[Category: Polymerase]]
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[[Category: Rna-dependent rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:48:03 2008''
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Current revision

The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 dead-end complex

PDB ID 1uvk

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