1lp1

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(New page: 200px<br /><applet load="1lp1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lp1, resolution 2.3&Aring;" /> '''Protein Z in complex ...)
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[[Image:1lp1.jpg|left|200px]]<br /><applet load="1lp1" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lp1, resolution 2.3&Aring;" />
 
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'''Protein Z in complex with an in vitro selected affibody'''<br />
 
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==Overview==
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==Protein Z in complex with an in vitro selected affibody==
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The broad binding repertoire of antibodies has permitted their use in a, wide range of applications. However, some uses of antibodies are precluded, due to limitations in the efficiency of antibody generation. In vitro, evolved binding proteins, selected from combinatorial libraries generated, around various alternative structural scaffolds, are promising, alternatives to antibodies. We have solved the crystal structure of a, complex of an all alpha-helical in vitro selected binding protein, (affibody) bound to protein Z, an IgG Fc-binding domain derived from, staphylococcal protein A. The structure of the complex reveals an extended, and complementary binding surface with similar properties to, protein-antibody interactions. The surface region of protein Z recognized, by the affibody is strikingly similar to the one used for IgG(1) Fc, binding, suggesting that this surface contains potential hot-spots for, binding. The implications of the selected affibody binding-mode for its, application as a universal binding protein are discussed.
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<StructureSection load='1lp1' size='340' side='right'caption='[[1lp1]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lp1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LP1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lp1 OCA], [https://pdbe.org/1lp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lp1 RCSB], [https://www.ebi.ac.uk/pdbsum/1lp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lp1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lp/1lp1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lp1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The broad binding repertoire of antibodies has permitted their use in a wide range of applications. However, some uses of antibodies are precluded due to limitations in the efficiency of antibody generation. In vitro evolved binding proteins, selected from combinatorial libraries generated around various alternative structural scaffolds, are promising alternatives to antibodies. We have solved the crystal structure of a complex of an all alpha-helical in vitro selected binding protein (affibody) bound to protein Z, an IgG Fc-binding domain derived from staphylococcal protein A. The structure of the complex reveals an extended and complementary binding surface with similar properties to protein-antibody interactions. The surface region of protein Z recognized by the affibody is strikingly similar to the one used for IgG(1) Fc binding, suggesting that this surface contains potential hot-spots for binding. The implications of the selected affibody binding-mode for its application as a universal binding protein are discussed.
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==About this Structure==
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Structural basis for recognition by an in vitro evolved affibody.,Hogbom M, Eklund M, Nygren PA, Nordlund P Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3191-6. Epub 2003 Feb 25. PMID:12604795<ref>PMID:12604795</ref>
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1LP1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with SO4 and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LP1 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for recognition by an in vitro evolved affibody., Hogbom M, Eklund M, Nygren PA, Nordlund P, Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3191-6. Epub 2003 Feb 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12604795 12604795]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1lp1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Eklund, M.]]
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[[Category: Eklund M]]
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[[Category: Hogbom, M.]]
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[[Category: Hogbom M]]
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[[Category: Nordlund, P.]]
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[[Category: Nordlund P]]
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[[Category: Nygren, P.A.]]
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[[Category: Nygren PA]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: affibody]]
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[[Category: in vitro evolved]]
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[[Category: protein-protein complex]]
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[[Category: three-helix bundle]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:44:30 2007''
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Current revision

Protein Z in complex with an in vitro selected affibody

PDB ID 1lp1

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