1tto

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{{Seed}}
 
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[[Image:1tto.png|left|200px]]
 
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==Crystal structure of the Rnase T1 variant R2==
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The line below this paragraph, containing "STRUCTURE_1tto", creates the "Structure Box" on the page.
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<StructureSection load='1tto' size='340' side='right'caption='[[1tto]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1tto]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TTO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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{{STRUCTURE_1tto| PDB=1tto | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tto OCA], [https://pdbe.org/1tto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tto RCSB], [https://www.ebi.ac.uk/pdbsum/1tto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tto ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNT1_ASPOR RNT1_ASPOR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tt/1tto_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tto ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ribonuclease T1 is an enzyme that cleaves single-stranded RNA with high specificity after guanylyl residues. Although this enzyme is a very good characterized protein with respect to structure and enzymatic function, we were only recently successful in generating RNase T1-RV, a variant where the specificity was changed from guanine to purine. As this change of substrate specificity was made at the cost of activity, the aim was now to further improve the overall activity of the enzyme. Therefore, we have substituted the tryptophan in position 59 by tyrosine. This substitution led to an increase of enzymatic activity in comparison to variant RV to 425%. As the extent of this enhancement is unique so far we have crystallized and analyzed the structure of this variant in order to get more insights into the reasons for this. Here, we present the crystal structure of this so-called RNase T1-R2 at 2.1A resolution. The structure was determined by molecular replacement using the coordinates of the RV variant (PDB entry: 1Q9E). The data were refined to an R-factor of 18.7% and R(free) of 24%, respectively. The asymmetric unit contains three molecules and the crystal packing is very similar to that of variant RV.
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===Crystal structure of the Rnase T1 variant R2===
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Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine.,Czaja R, Perbandt M, Betzel C, Hahn U Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:16157302<ref>PMID:16157302</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1tto" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16157302}}, adds the Publication Abstract to the page
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16157302 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16157302}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1TTO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTO OCA].
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==Reference==
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Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine., Czaja R, Perbandt M, Betzel C, Hahn U, Biochem Biophys Res Commun. 2005 Oct 28;336(3):882-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16157302 16157302]
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[[Category: Aspergillus oryzae]]
[[Category: Aspergillus oryzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Betzel, C.]]
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[[Category: Betzel C]]
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[[Category: Czaja, R.]]
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[[Category: Czaja R]]
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[[Category: Hahn, U.]]
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[[Category: Hahn U]]
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[[Category: Perbandt, M.]]
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[[Category: Perbandt M]]
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[[Category: Adenine specificity]]
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[[Category: Hydrolase]]
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[[Category: Ribonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:16:16 2008''
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Current revision

Crystal structure of the Rnase T1 variant R2

PDB ID 1tto

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OCA

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