1lqm

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(New page: 200px<br /><applet load="1lqm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lqm, resolution 3.20&Aring;" /> '''ESCHERICHIA COLI URA...)
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[[Image:1lqm.jpg|left|200px]]<br /><applet load="1lqm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lqm, resolution 3.20&Aring;" />
 
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'''ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN'''<br />
 
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==Overview==
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==ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN==
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The structures of a new crystal form of free Escherichia coli uracil DNA, glycosylase (UDG), containing four molecules in the asymmetric unit, and, two forms of its complex with the proteinaceous inhibitor Ugi, containing, two and four crystallographically independent complexes, have been, determined. A comparison of these structures and the already known crystal, structures containing UDG shows that the enzyme can be considered to be, made up of two independently moving structural entities or domains. A, detailed study of free and DNA-bound human enzyme strengthens this, conclusion. The domains close upon binding to uracil-containing DNA, whereas they do not appear to do so upon binding to Ugi. The comparative, study also shows that the mobility of the molecule involves the rigid-body, movement of the domains superposed on flexibility within domains.
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<StructureSection load='1lqm' size='340' side='right'caption='[[1lqm]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lqm]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_PBS2 Bacillus phage PBS2] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqm OCA], [https://pdbe.org/1lqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqm RCSB], [https://www.ebi.ac.uk/pdbsum/1lqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UNGI_BPPB2 UNGI_BPPB2] This protein binds specifically and reversibly to the host uracil-DNA glycosylase, preventing removal of uracil residues from PBS2 DNA by the host uracil-excision repair system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lqm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LQM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Phage_pbs1 Phage pbs1]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LQM OCA].
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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*[[Uracil glycosylase inhibitor|Uracil glycosylase inhibitor]]
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==Reference==
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__TOC__
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Domain closure and action of uracil DNA glycosylase (UDG): structures of new crystal forms containing the Escherichia coli enzyme and a comparative study of the known structures involving UDG., Saikrishnan K, Bidya Sagar M, Ravishankar R, Roy S, Purnapatre K, Handa P, Varshney U, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1269-76. Epub 2002, Jul 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12136137 12136137]
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</StructureSection>
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[[Category: Bacillus phage PBS2]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Phage pbs1]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Purnapatre K]]
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[[Category: Purnapatre, K.]]
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[[Category: Ravishankar R]]
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[[Category: Ravishankar, R.]]
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[[Category: Roy S]]
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[[Category: Roy, S.]]
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[[Category: Sagar MB]]
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[[Category: Sagar, M.B.]]
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[[Category: Saikrishnan K]]
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[[Category: Saikrishnan, K.]]
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[[Category: Varshney U]]
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[[Category: Varshney, U.]]
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[[Category: Vijayan M]]
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[[Category: Vijayan, M.]]
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[[Category: base excision]]
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[[Category: complex (hydrolase/inhibitor)]]
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[[Category: dna repair]]
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[[Category: glycosylase]]
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[[Category: inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:47:15 2007''
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Current revision

ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN

PDB ID 1lqm

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