2olw

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{{Seed}}
 
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[[Image:2olw.png|left|200px]]
 
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==Crystal Structure of E. coli pseudouridine synthase RluE==
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The line below this paragraph, containing "STRUCTURE_2olw", creates the "Structure Box" on the page.
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<StructureSection load='2olw' size='340' side='right'caption='[[2olw]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2olw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OLW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2olw| PDB=2olw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2olw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2olw OCA], [https://pdbe.org/2olw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2olw RCSB], [https://www.ebi.ac.uk/pdbsum/2olw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2olw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RLUE_ECOLI RLUE_ECOLI] Responsible for synthesis of pseudouridine from uracil-2457 in 23S ribosomal RNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/2olw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2olw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pseudouridine synthase RluE modifies U2457 in a stem of 23 S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. We determined the crystal structures of the C-terminal, catalytic domain of E. coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The protein has an active site cleft, conserved in all other pseudouridine synthases, that contains invariant Asp and Tyr residues implicated in catalysis. An electropositive surface patch that covers the active site cleft is just wide enough to accommodate an RNA stem. The RNA substrate stem can be docked to this surface such that the catalytic Asp is adjacent to the target base, and a conserved Arg is positioned to help flip the target base out of the stem into the enzyme active site. A flexible RluE specific loop lies close to the conserved region of the stem in the model, and may contribute to substrate specificity. The stem alone is not a good RluE substrate, suggesting RluE makes additional interactions with other regions in the ribosome.
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===Crystal Structure of E. coli pseudouridine synthase RluE===
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The crystal structure of E. coli rRNA pseudouridine synthase RluE.,Pan H, Ho JD, Stroud RM, Finer-Moore J J Mol Biol. 2007 Apr 13;367(5):1459-70. Epub 2007 Feb 7. PMID:17320904<ref>PMID:17320904</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2olw" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17320904}}, adds the Publication Abstract to the page
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*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17320904 is the PubMed ID number.
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*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_17320904}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2OLW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLW OCA].
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==Reference==
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The crystal structure of E. coli rRNA pseudouridine synthase RluE., Pan H, Ho JD, Stroud RM, Finer-Moore J, J Mol Biol. 2007 Apr 13;367(5):1459-70. Epub 2007 Feb 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17320904 17320904]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Finer-Moore, J.]]
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[[Category: Finer-Moore J]]
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[[Category: Ho, J D.]]
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[[Category: Ho JD]]
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[[Category: Pan, H.]]
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[[Category: Pan H]]
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[[Category: Stroud, R M.]]
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[[Category: Stroud RM]]
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[[Category: Bifurcated beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:29:21 2008''
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Current revision

Crystal Structure of E. coli pseudouridine synthase RluE

PDB ID 2olw

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