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1lss

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(New page: 200px<br /><applet load="1lss" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lss, resolution 2.30&Aring;" /> '''KTN Mja218 CRYSTAL S...)
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[[Image:1lss.jpg|left|200px]]<br /><applet load="1lss" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lss, resolution 2.30&Aring;" />
 
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'''KTN Mja218 CRYSTAL STRUCTURE IN COMPLEX WITH NAD+'''<br />
 
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==Overview==
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==KTN Mja218 CRYSTAL STRUCTURE IN COMPLEX WITH NAD+==
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The regulation of cation content is critical for cell growth. However, the, molecular mechanisms that gate the systems that control K+ movements, remain unclear. KTN is a highly conserved cytoplasmic domain present, ubiquitously in a variety of prokaryotic and eukaryotic K+ channels and, transporters. Here we report crystal structures for two representative KTN, domains that reveal a dimeric hinged assembly. Alternative ligands NAD+, and NADH block or vacate, respectively, the hinge region affecting the, dimer's conformational flexibility. Conserved, surface-exposed hydrophobic, patches that become coplanar upon hinge closure provide an assembly, interface for KTN tetramerization. Mutational analysis using the KefC, system demonstrates that this domain directly interacts with its, respective transmembrane constituent, coupling ligand-mediated KTN, conformational changes to the permease's activity.
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<StructureSection load='1lss' size='340' side='right'caption='[[1lss]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lss]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LSS FirstGlance]. <br>
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1LSS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LSS OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lss OCA], [https://pdbe.org/1lss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lss RCSB], [https://www.ebi.ac.uk/pdbsum/1lss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lss ProSAT]</span></td></tr>
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A mechanism of regulating transmembrane potassium flux through a ligand-mediated conformational switch., Roosild TP, Miller S, Booth IR, Choe S, Cell. 2002 Jun 14;109(6):781-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12086676 12086676]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRKA_METJA TRKA_METJA] Part of a potassium transport system (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1lss_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lss ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Single protein]]
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[[Category: Booth IR]]
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[[Category: Booth, I.R.]]
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[[Category: Choe S]]
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[[Category: Choe, S.]]
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[[Category: Miller S]]
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[[Category: Miller, S.]]
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[[Category: Roosild TP]]
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[[Category: Roosild, T.P.]]
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[[Category: NAD]]
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[[Category: ktn domain]]
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[[Category: ktra]]
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[[Category: nad]]
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[[Category: potassium channel]]
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[[Category: potassium transport]]
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[[Category: rck domain]]
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[[Category: rossman fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:50:57 2007''
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Current revision

KTN Mja218 CRYSTAL STRUCTURE IN COMPLEX WITH NAD+

PDB ID 1lss

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