1lsv

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(New page: 200px<br /><applet load="1lsv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lsv, resolution 2.40&Aring;" /> '''Crystal structure of...)
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[[Image:1lsv.gif|left|200px]]<br /><applet load="1lsv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lsv, resolution 2.40&Aring;" />
 
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'''Crystal structure of the CO-bound BjFixL heme domain'''<br />
 
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==Overview==
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==Crystal structure of the CO-bound BjFixL heme domain==
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Structures of the Bradyrhizobium japonicum FixL heme domain have been, determined in the absence and presence of specific ligands to elucidate, the detailed features of its O2 sensing mechanism. The putative roles of, spin-state and steric hindrance were evaluated by the structure, determination of ferrous CO-bound BjFixLH and correlating its features, with other ligand-bound structures. As found for NO-BjFixLH, no protein, conformational change was observed in CO-BjFixLH, suggesting a more, complicated mechanism than solely spin state or ligand sterics. To, evaluate the role of oxidation state, the structure of the ferrous, deoxy-BjFixLH was determined. The structure of deoxy-BjFixLH was found to, be virtually identical to the structure of the ferric met-BjFixLH. The, role of hydrogen bonding of substrates to a heme-pocket water was, evaluated by determining the structure of BjFixLH bound to, 1-methyl-imidazole that cannot form a hydrogen bond with this water. In, this case, the heme-mediated conformational change was observed, limiting, the potential importance of this interaction. Finally, the structure of, cyanomet-BjFixLH was revisited to rule out concerns regarding the partial, occupancy of the cyanide ligand in a previous structure. In the revised, structure, Arg 220 was found to move into the heme pocket to form a, hydrogen bond to the bound cyanide ligand. The implications of these, results on FixL's sensing mechanism are discussed.
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<StructureSection load='1lsv' size='340' side='right'caption='[[1lsv]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1lsv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LSV FirstGlance]. <br>
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1LSV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum] with HEM and CMO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LSV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lsv OCA], [https://pdbe.org/1lsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lsv RCSB], [https://www.ebi.ac.uk/pdbsum/1lsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lsv ProSAT]</span></td></tr>
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Structure-based mechanism of O2 sensing and ligand discrimination by the FixL heme domain of Bradyrhizobium japonicum., Hao B, Isaza C, Arndt J, Soltis M, Chan MK, Biochemistry. 2002 Oct 29;41(43):12952-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12390021 12390021]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIXL_BRADU FIXL_BRADU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ls/1lsv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lsv ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bradyrhizobium japonicum]]
[[Category: Bradyrhizobium japonicum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arndt, J.]]
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[[Category: Arndt J]]
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[[Category: Chan, M.K.]]
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[[Category: Chan MK]]
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[[Category: Hao, B.]]
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[[Category: Hao B]]
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[[Category: Isaza, C.]]
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[[Category: Isaza C]]
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[[Category: Soltis, M.]]
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[[Category: Soltis M]]
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[[Category: CMO]]
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[[Category: HEM]]
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[[Category: pas fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:51:06 2007''
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Current revision

Crystal structure of the CO-bound BjFixL heme domain

PDB ID 1lsv

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