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1lws

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(New page: 200px<br /><applet load="1lws" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lws, resolution 3.50&Aring;" /> '''Crystal structure of...)
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[[Image:1lws.gif|left|200px]]<br /><applet load="1lws" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lws, resolution 3.50&Aring;" />
 
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'''Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence'''<br />
 
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==Overview==
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==Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence==
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The first X-ray structures of an intein-DNA complex, that of the, two-domain homing endonuclease PI-SceI bound to its 36-base pair DNA, substrate, have been determined in the presence and absence of Ca(2+). The, DNA shows an asymmetric bending pattern, with a major 50 degree bend in, the endonuclease domain and a minor 22 degree bend in the splicing domain, region. Distortions of the DNA bound to the endonuclease domain cause the, insertion of the two cleavage sites in the catalytic center. DNA binding, induces changes in the protein conformation. The two overlapping, non-identical active sites in the endonucleolytic center contain two, Ca(+2) ions that coordinate to the catalytic Asp residues. Structure, analysis indicates that the top strand may be cleaved first.
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<StructureSection load='1lws' size='340' side='right'caption='[[1lws]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lws]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The November 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Inteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_11 10.2210/rcsb_pdb/mom_2010_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LWS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lws OCA], [https://pdbe.org/1lws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lws RCSB], [https://www.ebi.ac.uk/pdbsum/1lws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lws ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VATA_YEAST VATA_YEAST] Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase (vacuolar ATPase) is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mV, inside positive and acidic, in the vacuolar membrane vesicles. It may participate in maintenance of cytoplasmic Ca(2+) homeostasis. This is a catalytic subunit.<ref>PMID:1534148</ref> PI-SceI is an endonuclease that can cleave at a site present in a VMA1 allele that lacks the derived endonuclease segment of the open reading frame; cleavage at this site only occurs during meiosis and initiates "homing", a genetic event that converts a VMA1 allele lacking VDE into one that contains it.<ref>PMID:1534148</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lw/1lws_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lws ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LWS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LWS OCA].
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence., Moure CM, Gimble FS, Quiocho FA, Nat Struct Biol. 2002 Oct;9(10):764-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12219083 12219083]
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__TOC__
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</StructureSection>
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[[Category: Inteins]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Gimble FS]]
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[[Category: Gimble, F.S.]]
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[[Category: Moure CM]]
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[[Category: Moure, C.M.]]
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[[Category: Quiocho FA]]
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[[Category: Quiocho, F.A.]]
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[[Category: CA]]
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[[Category: endonuclease]]
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[[Category: homing endonuclease]]
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[[Category: intein]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:57:00 2007''
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Current revision

Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence

PDB ID 1lws

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