2z3s

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{{Seed}}
 
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[[Image:2z3s.png|left|200px]]
 
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==NMR structure of AgTx2-MTX==
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The line below this paragraph, containing "STRUCTURE_2z3s", creates the "Structure Box" on the page.
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<StructureSection load='2z3s' size='340' side='right'caption='[[2z3s]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2z3s]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z3S FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z3s OCA], [https://pdbe.org/2z3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z3s RCSB], [https://www.ebi.ac.uk/pdbsum/2z3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z3s ProSAT]</span></td></tr>
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{{STRUCTURE_2z3s| PDB=2z3s | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Agitoxin 2 (AgTx2) is a 38-residue scorpion toxin, cross-linked by three disulfide bridges, which acts on voltage-gated K(+) (Kv) channels. Maurotoxin (MTX) is a 34-residue scorpion toxin with an uncommon four-disulfide bridge reticulation, acting on both Ca(2+)-activated and Kv channels. A 39-mer chimeric peptide, named AgTx2-MTX, was designed from the sequence of the two toxins and chemically synthesized. It encompasses residues 1-5 of AgTx2, followed by the complete sequence of MTX. As established by enzyme cleavage, the new AgTx2-MTX molecule displays half-cystine pairings of the type C1-C5, C2-C6, C3-C7, and C4-C8, which is different from that of MTX. The 3D structure of AgTx2-MTX solved by (1)H-NMR, revealed both alpha-helical and beta-sheet structures, consistent with a common alpha/beta scaffold of scorpion toxins. Pharmacological assays of AgTx2-MTX revealed that this new molecule is more potent than both original toxins in blocking rat Kv1.2 channel. Docking simulations, performed with the 3D structure of AgTx2-MTX, confirmed this result and demonstrated the participation of the N-terminal domain of AgTx2 in its increased affinity for Kv1.2 through additional molecular contacts. Altogether, the data indicated that replacement of the N-terminal domain of MTX by the one of AgTx2 in the AgTx2-MTX chimera results in a reorganization of the disulfide bridge arrangement and an increase of affinity to the Kv1.2 channel.
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===NMR structure of AgTx2-MTX===
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Chemical synthesis and 1H-NMR 3D structure determination of AgTx2-MTX chimera, a new potential blocker for Kv1.2 channel, derived from MTX and AgTx2 scorpion toxins.,Pimentel C, M'Barek S, Visan V, Grissmer S, Sampieri F, Sabatier JM, Darbon H, Fajloun Z Protein Sci. 2008 Jan;17(1):107-18. Epub 2007 Nov 27. PMID:18042681<ref>PMID:18042681</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18042681}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2z3s" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18042681 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18042681}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2Z3S is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z3S OCA].
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[[Category: Darbon H]]
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[[Category: Fajloun Z]]
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==Reference==
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[[Category: Grissmer S]]
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Chemical synthesis and 1H-NMR 3D structure determination of AgTx2-MTX chimera, a new potential blocker for Kv1.2 channel, derived from MTX and AgTx2 scorpion toxins., Pimentel C, M'Barek S, Visan V, Grissmer S, Sampieri F, Sabatier JM, Darbon H, Fajloun Z, Protein Sci. 2008 Jan;17(1):107-18. Epub 2007 Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18042681 18042681]
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[[Category: M'Barrek S]]
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[[Category: Single protein]]
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[[Category: Pimentel C]]
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[[Category: Barrek, S M.]]
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[[Category: Sabatier JM]]
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[[Category: Darbon, H.]]
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[[Category: Visan V]]
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[[Category: Fajloun, Z.]]
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[[Category: Grissmer, S.]]
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[[Category: Pimentel, C.]]
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[[Category: Sabatier, J M.]]
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[[Category: Visan, V.]]
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[[Category: Agitoxin]]
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[[Category: Chimera]]
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[[Category: Inhibitory cystine knot]]
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[[Category: Maurotoxin]]
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[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:46:44 2008''
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Current revision

NMR structure of AgTx2-MTX

PDB ID 2z3s

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