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1lzo

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(New page: 200px<br /><applet load="1lzo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lzo, resolution 2.80&Aring;" /> '''Plasmodium Falciparu...)
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[[Image:1lzo.jpg|left|200px]]<br /><applet load="1lzo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lzo, resolution 2.80&Aring;" />
 
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'''Plasmodium Falciparum Triosephosphate Isomerase-Phosphoglycolate Complex'''<br />
 
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==Overview==
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==Plasmodium Falciparum Triosephosphate Isomerase-Phosphoglycolate Complex==
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Triosephosphate isomerase (TIM) has been the subject of many structural, and mechanistic studies. At position 96, there is a highly conserved Ser, residue, which is proximal to the catalytic site. Thus far, no specific, role has been ascribed to this residue. Plasmodium falciparum TIM (PfTIM), a fully catalytically active enzyme, is unique in possessing a Phe residue, at position 96. The structure of PfTIM complexed to phosphoglycolate (PG), a transition state analogue, has been determined in an effort to probe the, effects of the mutation at residue 96 on the nature of inhibitor-enzyme, interactions and the orientation of the critical catalytic loop (loop 6, residues 166-176) in TIM. Crystal structures of PfTIM complexed to, phosphoglycolate in orthorhombic (P2(1)2(1)2(1)) and monoclinic (C2) forms, were determined and refined at resolutions of 2.8 and 1.9 A, respectively., The P2(1)2(1)2(1) form contains two dimers in the asymmetric unit. In the, C2 form, the molecular and crystal 2-fold axes are coincident, leading to, a monomer in the asymmetric unit. The catalytic loop adopts the open state, in the P2(1)2(1)2(1) form and the closed conformation in the C2 crystal., The open conformation of the loop in the P2(1)2(1)2(1) form appears to be, a consequence of the Ser to Phe mutation at residue 96. The steric clash, between Phe96 and Ile172 probably impedes loop closure in PfTIM-ligand, complexes. The PfTIM-PG complex is the first example of a TIM-ligand, complex being observed in both loop open and closed forms. In the C2 form, (loop closed), Phe96 and Leu167 adopt alternative conformations that are, different from the ones observed in the open form, permitting loop, closure. These structures provide strong support for the view that loop, closure is not essential for ligand binding and that dynamic loop movement, may occur in both free and ligand-bound forms of the enzyme.
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<StructureSection load='1lzo' size='340' side='right'caption='[[1lzo]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lzo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LZO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGA:2-PHOSPHOGLYCOLIC+ACID'>PGA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lzo OCA], [https://pdbe.org/1lzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lzo RCSB], [https://www.ebi.ac.uk/pdbsum/1lzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lzo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TPIS_PLAFA TPIS_PLAFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/1lzo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lzo ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LZO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum] with PGA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LZO OCA].
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*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the Plasmodium falciparum triosephosphate isomerase-phosphoglycolate complex in two crystal forms: characterization of catalytic loop open and closed conformations in the ligand-bound state., Parthasarathy S, Ravindra G, Balaram H, Balaram P, Murthy MR, Biochemistry. 2002 Nov 5;41(44):13178-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12403619 12403619]
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[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
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[[Category: Single protein]]
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[[Category: Balaram H]]
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[[Category: Triose-phosphate isomerase]]
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[[Category: Balaram P]]
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[[Category: Balaram, H.]]
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[[Category: Murthy MR]]
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[[Category: Balaram, P.]]
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[[Category: Parthasarathy S]]
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[[Category: Murthy, M.R.]]
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[[Category: Parthasarathy, S.]]
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[[Category: PGA]]
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[[Category: triosephosphate isomerase; phosphoglycolate; loop dynamics; loop open conformation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:00:55 2007''
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Current revision

Plasmodium Falciparum Triosephosphate Isomerase-Phosphoglycolate Complex

PDB ID 1lzo

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