1lzw

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(New page: 200px<br /><applet load="1lzw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lzw, resolution 2.50&Aring;" /> '''Structural basis of ...)
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[[Image:1lzw.jpg|left|200px]]<br /><applet load="1lzw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lzw, resolution 2.50&Aring;" />
 
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'''Structural basis of ClpS-mediated switch in ClpA substrate recognition'''<br />
 
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==Overview==
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==Structural basis of ClpS-mediated switch in ClpA substrate recognition==
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In Escherichia coli, protein degradation is performed by several, proteolytic machines, including ClpAP. Generally, the substrate, specificity of these machines is determined by chaperone components, such, as ClpA. In some cases, however, the specificity is modified by adaptor, proteins, such as ClpS. Here we report the 2.5 A resolution crystal, structure of ClpS in complex with the N-terminal domain of ClpA. Using, mutagenesis, we demonstrate that two contact residues (Glu79 and Lys 84), are essential not only for ClpAS complex formation but also for, ClpAPS-mediated substrate degradation. The corresponding residues are, absent in the chaperone ClpB, providing a structural rationale for the, unique specificity shown by ClpS despite the high overall similarity, between ClpA and ClpB. To determine the location of ClpS within the ClpA, hexamer, we modeled the N-terminal domain of ClpA onto a structurally, defined, homologous AAA+ protein. From this model, we proposed a molecular, mechanism to explain the ClpS-mediated switch in ClpA substrate, specificity.
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<StructureSection load='1lzw' size='340' side='right'caption='[[1lzw]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lzw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LZW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lzw OCA], [https://pdbe.org/1lzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lzw RCSB], [https://www.ebi.ac.uk/pdbsum/1lzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lzw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CLPS_ECOLI CLPS_ECOLI] Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/1lzw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lzw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LZW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LZW OCA].
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*[[Heat Shock Protein structures|Heat Shock Protein structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA., Zeth K, Ravelli RB, Paal K, Cusack S, Bukau B, Dougan DA, Nat Struct Biol. 2002 Dec;9(12):906-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12426582 12426582]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Bukau, B.]]
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[[Category: Bukau B]]
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[[Category: Cusack, S.]]
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[[Category: Cusack S]]
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[[Category: Dougan, D.A.]]
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[[Category: Dougan DA]]
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[[Category: Paal, K.]]
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[[Category: Paal K]]
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[[Category: Ravelli, R.B.]]
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[[Category: Ravelli RB]]
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[[Category: Zeth, K.]]
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[[Category: Zeth K]]
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[[Category: PT]]
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[[Category: alpha-beta-protein (clps)]]
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[[Category: alpha-protein (clpa-nd)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:01:14 2007''
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Current revision

Structural basis of ClpS-mediated switch in ClpA substrate recognition

PDB ID 1lzw

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