1nzi

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{{Seed}}
 
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[[Image:1nzi.png|left|200px]]
 
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==Crystal Structure of the CUB1-EGF Interaction Domain of Complement Protease C1s==
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The line below this paragraph, containing "STRUCTURE_1nzi", creates the "Structure Box" on the page.
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<StructureSection load='1nzi' size='340' side='right'caption='[[1nzi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nzi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NZI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1nzi| PDB=1nzi | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nzi OCA], [https://pdbe.org/1nzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nzi RCSB], [https://www.ebi.ac.uk/pdbsum/1nzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nzi ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/C1S_HUMAN C1S_HUMAN] Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:[https://omim.org/entry/613783 613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
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== Function ==
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[https://www.uniprot.org/uniprot/C1S_HUMAN C1S_HUMAN] C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/1nzi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nzi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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C1, the complex that triggers the classical pathway of complement, is assembled from two modular proteases C1r and C1s and a recognition protein C1q. The N-terminal CUB1-EGF segments of C1r and C1s are key elements of the C1 architecture, because they mediate both Ca2+-dependent C1r-C1s association and interaction with C1q. The crystal structure of the interaction domain of C1s has been solved and refined to 1.5 A resolution. The structure reveals a head-to-tail homodimer involving interactions between the CUB1 module of one monomer and the epidermal growth factor (EGF) module of its counterpart. A Ca2+ ion is bound to each EGF module and stabilizes both the intra- and inter-monomer interfaces. Unexpectedly, a second Ca2+ ion is bound to the distal end of each CUB1 module, through six ligands contributed by Glu45, Asp53, Asp98, and two water molecules. These acidic residues and Tyr17 are conserved in approximately two-thirds of the CUB repertoire and define a novel, Ca2+-binding CUB module subset. The C1s structure was used to build a model of the C1r-C1s CUB1-EGF heterodimer, which in C1 connects C1r to C1s and mediates interaction with C1q. A structural model of the C1q/C1r/C1s interface is proposed, where the rod-like collagen triple helix of C1q is accommodated into a groove along the transversal axis of the C1r-C1s heterodimer.
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===Crystal Structure of the CUB1-EGF Interaction Domain of Complement Protease C1s===
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X-ray structure of the Ca2+-binding interaction domain of C1s. Insights into the assembly of the C1 complex of complement.,Gregory LA, Thielens NM, Arlaud GJ, Fontecilla-Camps JC, Gaboriaud C J Biol Chem. 2003 Aug 22;278(34):32157-64. Epub 2003 Jun 4. PMID:12788922<ref>PMID:12788922</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12788922}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1nzi" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12788922 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12788922}}
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__TOC__
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</StructureSection>
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==Disease==
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Known disease associated with this structure: C1r/C1s deficiency, combined OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=120580 120580]], C1s deficiency, isolated OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=120580 120580]]
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==About this Structure==
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1NZI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZI OCA].
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==Reference==
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X-ray structure of the Ca2+-binding interaction domain of C1s. Insights into the assembly of the C1 complex of complement., Gregory LA, Thielens NM, Arlaud GJ, Fontecilla-Camps JC, Gaboriaud C, J Biol Chem. 2003 Aug 22;278(34):32157-64. Epub 2003 Jun 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12788922 12788922]
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[[Category: Complement subcomponent C1s]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arlaud, G J.]]
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[[Category: Arlaud GJ]]
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[[Category: Fontecilla-Camps, J C.]]
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[[Category: Fontecilla-Camps JC]]
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[[Category: Gaboriaud, C.]]
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[[Category: Gaboriaud C]]
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[[Category: Gregory, L A.]]
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[[Category: Gregory LA]]
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[[Category: Thielens, N M.]]
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[[Category: Thielens NM]]
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[[Category: Calcium]]
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[[Category: Complement]]
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[[Category: Cub]]
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[[Category: Egf]]
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[[Category: Innate immunity]]
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[[Category: Modular structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:08:40 2008''
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Current revision

Crystal Structure of the CUB1-EGF Interaction Domain of Complement Protease C1s

PDB ID 1nzi

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