1m07

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(New page: 200px<br /><applet load="1m07" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m07, resolution 1.80&Aring;" /> '''RESIDUES INVOLVED IN...)
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[[Image:1m07.gif|left|200px]]<br /><applet load="1m07" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m07, resolution 1.80&Aring;" />
 
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'''RESIDUES INVOLVED IN THE CATALYSIS AND BASE SPECIFICITY OF CYTOTOXIC RIBONUCLEASE FROM BULLFROG (RANA CATESBEIANA)'''<br />
 
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==Overview==
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==RESIDUES INVOLVED IN THE CATALYSIS AND BASE SPECIFICITY OF CYTOTOXIC RIBONUCLEASE FROM BULLFROG (RANA CATESBEIANA)==
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The Rana catesbeiana (bullfrog) ribonucleases, which belong to the RNase A, superfamily, exert cytotoxicity toward tumor cells. RC-RNase, the most, active among frog ribonucleases, has a unique base preference for, pyrimidine-guanine rather than pyrimidine-adenine in RNase A. Residues of, RC-RNase involved in base specificity and catalytic activity were, determined by site-directed mutagenesis, k(cat)/K(m) analysis toward, dinucleotides, and cleavage site analysis of RNA substrate. The results, show that Pyr-1 (N-terminal pyroglutamate), Lys-9, and Asn-38 along with, His-10, Lys-35, and His-103 are involved in catalytic activity, whereas, Pyr-1, Thr-39, Thr-70, Lys-95, and Glu-97 are involved in base, specificity. The cytotoxicity of RC-RNase is correlated, but not, proportional to, its catalytic activity. The crystal structure of the, RC-RNase.d(ACGA) complex was determined at 1.80 A resolution. Residues, Lys-9, His-10, Lys-35, and His-103 interacted directly with catalytic, phosphate at the P(1) site, and Lys-9 was stabilized by hydrogen bonds, contributed by Pyr-1, Tyr-28, and Asn-38. Thr-70 acts as a hydrogen bond, donor for cytosine through Thr-39 and determines B(1) base specificity., Interestingly, Pyr-1 along with Lys-95 and Glu-97 form four hydrogen bonds, with guanine at B(2) site and determine B(2) base specificity.
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<StructureSection load='1m07' size='340' side='right'caption='[[1m07]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m07]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lithobates_catesbeianus Lithobates catesbeianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M07 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m07 OCA], [https://pdbe.org/1m07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m07 RCSB], [https://www.ebi.ac.uk/pdbsum/1m07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m07 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNASO_LITCT RNASO_LITCT] Preferentially cleaves single-stranded RNA at pyrimidine residues with a 3'flanking guanine. Hydrolyzes poly(U) and poly(C) as substrates, and prefers the former. The S-lectins in frog eggs may be involved in the fertilization and development of the frog embryo. This lectin agglutinates various animal cells, including normal lymphocytes, erythrocytes, and fibroblasts of animal and human origin. It is cytotoxic against several tumor cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m0/1m07_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m07 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1M07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M07 OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Residues involved in the catalysis, base specificity, and cytotoxicity of ribonuclease from Rana catesbeiana based upon mutagenesis and X-ray crystallography., Leu YJ, Chern SS, Wang SC, Hsiao YY, Amiraslanov I, Liaw YC, Liao YD, J Biol Chem. 2003 Feb 28;278(9):7300-9. Epub 2002 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12499382 12499382]
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[[Category: Large Structures]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Lithobates catesbeianus]]
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[[Category: Rana catesbeiana]]
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[[Category: Amiraslanov I]]
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[[Category: Single protein]]
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[[Category: Chern S-S]]
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[[Category: Amiraslanov, I.]]
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[[Category: Hsiao Y-Y]]
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[[Category: Chern, S.S.]]
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[[Category: Leu Y-J]]
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[[Category: Hsiao, Y.Y.]]
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[[Category: Liao Y-D]]
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[[Category: Leu, Y.J.]]
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[[Category: Liaw Y-C]]
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[[Category: Liao, Y.D.]]
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[[Category: Wang S-C]]
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[[Category: Liaw, Y.C.]]
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[[Category: Wang, S.C.]]
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[[Category: bullfrog]]
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[[Category: cytotoxicity]]
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[[Category: rc-rnase-d(acga)]]
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[[Category: ribonuclease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:01:42 2007''
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Current revision

RESIDUES INVOLVED IN THE CATALYSIS AND BASE SPECIFICITY OF CYTOTOXIC RIBONUCLEASE FROM BULLFROG (RANA CATESBEIANA)

PDB ID 1m07

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