2i4s

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{{Seed}}
 
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[[Image:2i4s.png|left|200px]]
 
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==PDZ domain of EpsC from Vibrio cholerae, residues 204-305==
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The line below this paragraph, containing "STRUCTURE_2i4s", creates the "Structure Box" on the page.
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<StructureSection load='2i4s' size='340' side='right'caption='[[2i4s]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2i4s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I4S FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epsC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
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{{STRUCTURE_2i4s| PDB=2i4s | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i4s OCA], [https://pdbe.org/2i4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i4s RCSB], [https://www.ebi.ac.uk/pdbsum/2i4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i4s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/GSPC_VIBCH GSPC_VIBCH]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins (By similarity). Required for secretion of cholera toxin through the outer membrane.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/2i4s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i4s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The type 2 secretion system (T2SS) occurring in Gram-negative bacteria is composed of 12-15 different proteins which form large assemblies spanning two membranes and secreting several virulence factors in folded state across the outer membrane. The T2SS component EpsC of Vibrio cholerae plays an important role in this machinery. While anchored in the inner membrane, by far the largest part of EpsC is periplasmic, containing a so-called homology region (HR) domain and a PDZ domain. Here we report studies on the structure and function of both periplasmic domains of EpsC. The crystal structures of two variants of the PDZ domain of EpsC from V. cholerae were determined at better than 2 A resolution. Compared to the short variant, the longer variant contains an additional N-terminal helix, and reveals a significant difference in the position of helix alphaB with respect to the beta-sheet. Both our structures show that the PDZ domain of EpsC adopts a more open form than in previously reported structures of other PDZ domains. Most interestingly, in the crystals of the short EpsC-PDZ domain the peptide binding groove interacts with an alpha-helix from a neighboring subunit burying approximately 921 A2 solvent accessible surface. This makes it possible that the PDZ domain of this bacterial protein binds proteins in a manner which is altogether different from that seen in any other PDZ domain so far. We also determined that the HR domain of EpsC is primarily responsible for the interaction with the secretin EpsD, while the PDZ is not, or much less, so. This new finding, together with studies of others, leads to the suggestion that the PDZ domain of EpsC may interact with exoproteins to be secreted while the HR domain plays a key role in linking the inner-membrane sub-complex of the T2SS in V. cholerae to the outer membrane secretin.
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===PDZ domain of EpsC from Vibrio cholerae, residues 204-305===
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Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio cholerae.,Korotkov KV, Krumm B, Bagdasarian M, Hol WG J Mol Biol. 2006 Oct 20;363(2):311-21. Epub 2006 Aug 18. PMID:16978643<ref>PMID:16978643</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2i4s" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16978643}}, adds the Publication Abstract to the page
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*[[General secretion pathway protein 3D structures|General secretion pathway protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16978643 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16978643}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillo virgola del koch trevisan 1884]]
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2I4S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I4S OCA].
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[[Category: Large Structures]]
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[[Category: Bagdasarian, M]]
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==Reference==
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[[Category: Hol, W G.J]]
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Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio cholerae., Korotkov KV, Krumm B, Bagdasarian M, Hol WG, J Mol Biol. 2006 Oct 20;363(2):311-21. Epub 2006 Aug 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16978643 16978643]
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[[Category: Korotkov, K V]]
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[[Category: Single protein]]
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[[Category: Krumm, B]]
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[[Category: Vibrio cholerae]]
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[[Category: Bagdasarian, M.]]
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[[Category: Hol, W G.J.]]
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[[Category: Korotkov, K V.]]
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[[Category: Krumm, B.]]
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[[Category: Epsc]]
[[Category: Epsc]]
[[Category: General secretion pathway]]
[[Category: General secretion pathway]]
[[Category: Gspc]]
[[Category: Gspc]]
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[[Category: Membrane protein]]
[[Category: Pdz domain]]
[[Category: Pdz domain]]
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[[Category: Protein transport]]
[[Category: Type 2 secretion system]]
[[Category: Type 2 secretion system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:15:52 2008''
 

Current revision

PDZ domain of EpsC from Vibrio cholerae, residues 204-305

PDB ID 2i4s

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