1s94

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{{Seed}}
 
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[[Image:1s94.png|left|200px]]
 
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==Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei==
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The line below this paragraph, containing "STRUCTURE_1s94", creates the "Structure Box" on the page.
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<StructureSection load='1s94' size='340' side='right'caption='[[1s94]], [[Resolution|resolution]] 3.34&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s94]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Doryteuthis_pealeii Doryteuthis pealeii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S94 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.34&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s94 OCA], [https://pdbe.org/1s94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s94 RCSB], [https://www.ebi.ac.uk/pdbsum/1s94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s94 ProSAT]</span></td></tr>
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{{STRUCTURE_1s94| PDB=1s94 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O46345_DORPE O46345_DORPE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s9/1s94_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s94 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Intracellular membrane fusion processes are mediated by the spatial and temporal control of SNARE complex assembly that results in the formation of a four-helical bundle, composed of one vesicle SNARE and three target membrane SNARE polypeptide chains. Syntaxins are essential t-SNAREs and are characterized by an N-terminal Habc domain, a flexible linker region, a coiled-coil or SNARE motif and a membrane anchor. The N-terminal Habc domain fulfills important regulatory functions while the coiled-coil motif, present in all SNAREs, is sufficient for SNARE complex formation, which is thought to drive membrane fusion. RESULTS: Here we report the crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei, s-syntaxin. Squid Habc crystallizes as a dimer and the monomer structure consists of a three-helical bundle. One molecule is strikingly similar to mammalian syntaxin 1A while the second one shows a structural deviation from the common fold in that the C-terminal part of helix C unwinds and adopts an extended conformation. CONCLUSION: Conservation of surface residues indicates that the cytosolic part of s-syntaxin can adopt an auto-inhibitory closed conformation that may bind squid neuronal Sec1, s-Sec1, in the same manner as observed in structure of the rat nSec1/syntaxin 1A complex. Furthermore, despite the overall structural similarity, the observed changes at the C-terminus of one molecule indicate structural plasticity in neuronal syntaxin. Implications of the structural conservation and the changes are discussed with respect to potential Habc domain binding partners such as Munc13, which facilitates the transition from the closed to the open conformation.
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===Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei===
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Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei reveals conformational plasticity at its C-terminus.,Bracher A, Weissenhorn W BMC Struct Biol. 2004 Mar 15;4:6. PMID:15113421<ref>PMID:15113421</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1s94" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15113421}}, adds the Publication Abstract to the page
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*[[Syntaxin 3D structures|Syntaxin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15113421 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15113421}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Doryteuthis pealeii]]
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1S94 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Loligo_pealei Loligo pealei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S94 OCA].
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[[Category: Large Structures]]
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[[Category: Bracher A]]
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==Reference==
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[[Category: Weissenhorn W]]
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Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei reveals conformational plasticity at its C-terminus., Bracher A, Weissenhorn W, BMC Struct Biol. 2004 Mar 15;4:6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15113421 15113421]
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[[Category: Loligo pealei]]
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[[Category: Single protein]]
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[[Category: Bracher, A.]]
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[[Category: Weissenhorn, W.]]
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[[Category: Structural plasticity]]
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[[Category: Three helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:16:06 2008''
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Current revision

Crystal structure of the Habc domain of neuronal syntaxin from the squid Loligo pealei

PDB ID 1s94

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