1m3x

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(New page: 200px<br /><applet load="1m3x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m3x, resolution 2.55&Aring;" /> '''Photosynthetic React...)
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[[Image:1m3x.gif|left|200px]]<br /><applet load="1m3x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m3x, resolution 2.55&Aring;" />
 
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'''Photosynthetic Reaction Center From Rhodobacter Sphaeroides'''<br />
 
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==Overview==
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==Photosynthetic Reaction Center From Rhodobacter Sphaeroides==
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The structure of the reaction center from Rhodobacter sphaeroides has been, solved by using x-ray diffraction at a 2.55-A resolution limit. Three, lipid molecules that lie on the surface of the protein are resolved in the, electron density maps. In addition to a cardiolipin that has previously, been reported [McAuley, K. E., Fyfe, P. K., Ridge, J. P., Isaacs, N. W., Cogdell, R. J. &amp; Jones, M. R. (1999) Proc. Natl. Acad. Sci. USA 96, 14706-14711], two other major lipids of the cell membrane are found, a, phosphatidylcholine and a glucosylgalactosyl diacylglycerol. The presence, of these three lipids has been confirmed by laser mass spectroscopy. The, lipids are located in the hydrophobic region of the protein surface and, interact predominately with hydrophobic amino acids, in particular, aromatic residues. Although the cardiolipin is over 15 A from the, cofactors, the other two lipids are in close contact with the cofactors, and may contribute to the difference in energetics for the two branches of, cofactors that is primarily responsible for the asymmetry of electron, transfer. The glycolipid is 3.5 A from the active bacteriochlorophyll, monomer and shields this cofactor from the solvent in contrast to a much, greater exposed surface evident for the inactive bacteriochlorophyll, monomer. The phosphate atom of phosphatidylcholine is 6.5 A from the, inactive bacteriopheophytin, and the associated electrostatic interactions, may contribute to electron transfer rates involving this cofactor., Overall, the lipids span a distance of approximately 30 A, which is, consistent with a bilayer-like arrangement suggesting the presence of an, "inner shell" of lipids around membrane proteins that is critical for, membrane function.
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<StructureSection load='1m3x' size='340' side='right'caption='[[1m3x]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1m3x]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M3X FirstGlance]. <br>
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1M3X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE, CL, BCL, BPH, U10, SPO, CDL, PC1 and GGD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M3X OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GGD:NONADEC-10-ENOIC+ACID+2-[3,4-DIHYDROXY-6-HYDROXYMETHYL-5-(3,4,5-TRIHYDROXY-6-HYDROXYMETHYL-TETRAHYDRO-PYRAN-2-YLOXY)-TETRAHYDRO-PYRAN-2-YLOXY]-1-OCTADEC-9-ENOYLOXYMETHYL-ETHYL+ESTER'>GGD</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene>, <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m3x OCA], [https://pdbe.org/1m3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m3x RCSB], [https://www.ebi.ac.uk/pdbsum/1m3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m3x ProSAT]</span></td></tr>
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Interactions between lipids and bacterial reaction centers determined by protein crystallography., Camara-Artigas A, Brune D, Allen JP, Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11055-60. Epub 2002 Aug 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12167672 12167672]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Rhodobacter sphaeroides]]
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[https://www.uniprot.org/uniprot/RCEL_CERSP RCEL_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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[[Category: Allen, J.P.]]
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== Evolutionary Conservation ==
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[[Category: Brune, D.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Camara-Artigas, A.]]
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Check<jmol>
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[[Category: BCL]]
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<jmolCheckbox>
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[[Category: BPH]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m3/1m3x_consurf.spt"</scriptWhenChecked>
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[[Category: CDL]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: CL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: FE]]
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</jmolCheckbox>
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[[Category: GGD]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m3x ConSurf].
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[[Category: PC1]]
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<div style="clear:both"></div>
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[[Category: SPO]]
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__TOC__
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[[Category: U10]]
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</StructureSection>
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[[Category: alpha helix]]
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[[Category: Cereibacter sphaeroides]]
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[[Category: membrane protein]]
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[[Category: Large Structures]]
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[[Category: Allen JP]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:07:31 2007''
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[[Category: Brune D]]
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[[Category: Camara-Artigas A]]

Current revision

Photosynthetic Reaction Center From Rhodobacter Sphaeroides

PDB ID 1m3x

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