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1m4i

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(New page: 200px<br /><applet load="1m4i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m4i, resolution 1.5&Aring;" /> '''Aminoglycoside 2'-N-a...)
Current revision (07:39, 14 February 2024) (edit) (undo)
 
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[[Image:1m4i.jpg|left|200px]]<br /><applet load="1m4i" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m4i, resolution 1.5&Aring;" />
 
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'''Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-Complex with Coenzyme A and Kanamycin A'''<br />
 
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==Overview==
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==Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-Complex with Coenzyme A and Kanamycin A==
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AAC(2')-Ic catalyzes the coenzyme A (CoA)-dependent acetylation of the 2', hydroxyl or amino group of a broad spectrum of aminoglycosides. The, crystal structure of the AAC(2')-Ic from Mycobacterium tuberculosis has, been determined in the apo enzyme form and in ternary complexes with CoA, and either tobramycin, kanamycin A or ribostamycin, representing the first, structures of an aminoglycoside acetyltransferase bound to a drug. The, overall fold of AAC(2')-Ic places it in the GCN5-related, N-acetyltransferase (GNAT) superfamily. Although the physiological, function of AAC(2')-Ic is uncertain, a structural analysis of these, high-affinity aminoglycoside complexes suggests that the enzyme may, acetylate a key biosynthetic intermediate of mycothiol, the major reducing, agent in mycobacteria, and participate in the regulation of cellular redox, potential.
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<StructureSection load='1m4i' size='340' side='right'caption='[[1m4i]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1m4i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M4I FirstGlance]. <br>
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1M4I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with COA, KAN and PAP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M4I OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KAN:KANAMYCIN+A'>KAN</scene>, <scene name='pdbligand=PAP:3-PHOSPHATE-ADENOSINE-5-DIPHOSPHATE'>PAP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m4i OCA], [https://pdbe.org/1m4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m4i RCSB], [https://www.ebi.ac.uk/pdbsum/1m4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m4i ProSAT]</span></td></tr>
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Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates., Vetting MW, Hegde SS, Javid-Majd F, Blanchard JS, Roderick SL, Nat Struct Biol. 2002 Sep;9(9):653-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12161746 12161746]
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</table>
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[[Category: Mycobacterium tuberculosis]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/AAC2_MYCTU AAC2_MYCTU] Catalyzes the coenzyme A-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides. It confers resistance to aminoglycosides.
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[[Category: Blanchard, J.S.]]
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== Evolutionary Conservation ==
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[[Category: Hegde, S.S.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Javid-Majd, F.]]
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Check<jmol>
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[[Category: Roderick, S.L.]]
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<jmolCheckbox>
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[[Category: Vetting, M.W.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m4/1m4i_consurf.spt"</scriptWhenChecked>
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[[Category: COA]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: KAN]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: PAP]]
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</jmolCheckbox>
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[[Category: coa binding motif]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m4i ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:08:24 2007''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Blanchard JS]]
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[[Category: Hegde SS]]
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[[Category: Javid-Majd F]]
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[[Category: Roderick SL]]
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[[Category: Vetting MW]]

Current revision

Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-Complex with Coenzyme A and Kanamycin A

PDB ID 1m4i

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