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1m5z

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(New page: 200px<br /><applet load="1m5z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5z" /> '''The PDZ7 of Glutamate Receptor Interacting P...)
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[[Image:1m5z.jpg|left|200px]]<br /><applet load="1m5z" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m5z" />
 
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'''The PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area'''<br />
 
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==Overview==
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==The PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area==
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Glutamate receptor interacting protein 1 (GRIP1) is a scaffold protein, composed of seven PDZ (Postsynaptic synaptic density-95/Discs large/Zona, occludens-1) domains. The protein plays important roles in the synaptic, targeting of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, (AMPA) receptors. The interaction between GRIP1 PDZ7 and a Ras guanine, nucleotide exchange factor, GRASP-1, regulates synaptic distribution of, AMPA receptors. Here, we describe the three-dimensional structure of GRIP1, PDZ7 determined by NMR spectroscopy. GRIP1 PDZ7 contains a closed carboxyl, group-binding pocket and a narrow alphaB/betaB-groove that is not likely, to bind to classical PDZ ligands. Unexpectedly, GRIP1 PDZ7 contains a, large solvent-exposed hydrophobic surface at a site distinct from the, conventional ligand-binding alphaB/betaB-groove. NMR titration experiments, show that GRIP1 PDZ7 binds to GRASP-1 via this hydrophobic surface. Our, data uncover a novel PDZ domain-mediated protein interaction mode that may, be responsible for multimerization of other PDZ domain-containing scaffold, proteins.
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<StructureSection load='1m5z' size='340' side='right'caption='[[1m5z]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m5z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M5Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m5z OCA], [https://pdbe.org/1m5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m5z RCSB], [https://www.ebi.ac.uk/pdbsum/1m5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m5z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GRIP1_RAT GRIP1_RAT] May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.<ref>PMID:9069286</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/1m5z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m5z ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glutamate receptor interacting protein 1 (GRIP1) is a scaffold protein composed of seven PDZ (Postsynaptic synaptic density-95/Discs large/Zona occludens-1) domains. The protein plays important roles in the synaptic targeting of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. The interaction between GRIP1 PDZ7 and a Ras guanine nucleotide exchange factor, GRASP-1, regulates synaptic distribution of AMPA receptors. Here, we describe the three-dimensional structure of GRIP1 PDZ7 determined by NMR spectroscopy. GRIP1 PDZ7 contains a closed carboxyl group-binding pocket and a narrow alphaB/betaB-groove that is not likely to bind to classical PDZ ligands. Unexpectedly, GRIP1 PDZ7 contains a large solvent-exposed hydrophobic surface at a site distinct from the conventional ligand-binding alphaB/betaB-groove. NMR titration experiments show that GRIP1 PDZ7 binds to GRASP-1 via this hydrophobic surface. Our data uncover a novel PDZ domain-mediated protein interaction mode that may be responsible for multimerization of other PDZ domain-containing scaffold proteins.
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==About this Structure==
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PDZ7 of glutamate receptor interacting protein binds to its target via a novel hydrophobic surface area.,Feng W, Fan JS, Jiang M, Shi YW, Zhang M J Biol Chem. 2002 Oct 25;277(43):41140-6. Epub 2002 Aug 23. PMID:12196542<ref>PMID:12196542</ref>
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1M5Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M5Z OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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PDZ7 of glutamate receptor interacting protein binds to its target via a novel hydrophobic surface area., Feng W, Fan JS, Jiang M, Shi YW, Zhang M, J Biol Chem. 2002 Oct 25;277(43):41140-6. Epub 2002 Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12196542 12196542]
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</div>
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<div class="pdbe-citations 1m5z" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Fan J]]
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[[Category: Fan, J.]]
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[[Category: Feng W]]
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[[Category: Feng, W.]]
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[[Category: Jiang M]]
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[[Category: Jiang, M.]]
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[[Category: Shi Y]]
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[[Category: Shi, Y.]]
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[[Category: Zhang M]]
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[[Category: Zhang, M.]]
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[[Category: six beta-strands and two alpha-helices]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:10:21 2007''
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Current revision

The PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area

PDB ID 1m5z

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