1m9n

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(New page: 200px<br /><applet load="1m9n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m9n, resolution 1.93&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1m9n.jpg|left|200px]]<br /><applet load="1m9n" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m9n, resolution 1.93&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE HOMODIMERIC BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME AVIAN ATIC IN COMPLEX WITH AICAR AND XMP AT 1.93 ANGSTROMS.'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE HOMODIMERIC BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME AVIAN ATIC IN COMPLEX WITH AICAR AND XMP AT 1.93 ANGSTROMS.==
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ATIC encompasses both AICAR transformylase and IMP cyclohydrolase, activities that are responsible for the catalysis of the penultimate and, final steps of the purine de novo synthesis pathway. The formyl transfer, reaction catalyzed by the AICAR Tfase domain is substantially more, demanding than that catalyzed by the other folate-dependent enzyme of the, purine biosynthesis pathway, GAR transformylase. Identification of the, AICAR Tfase active site and key catalytic residues is essential to, elucidate how the non-nucleophilic AICAR amino group is activated for, formyl transfer. Hence, the crystal structure of dimeric avian ATIC was, determined as a complex with the AICAR Tfase substrate AICAR, as well as, with an IMP cyclohydrolase inhibitor, XMP, to 1.93 A resolution. AICAR is, bound at the dimer interface of the transformylase domains and forms an, extensive hydrogen bonding network with a multitude of active site, residues. The crystal structure suggests that the conformation of the, 4-carboxamide of AICAR is poised to increase the nucleophilicity of the C5, amine, while proton abstraction occurs via His(268) concomitant with, formyl transfer. Lys(267) is likely to be involved in the stabilization of, the anionic formyl transfer transition state and in subsequent protonation, of the THF leaving group.
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<StructureSection load='1m9n' size='340' side='right'caption='[[1m9n]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m9n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMZ:AMINOIMIDAZOLE+4-CARBOXAMIDE+RIBONUCLEOTIDE'>AMZ</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=XMP:XANTHOSINE-5-MONOPHOSPHATE'>XMP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9n OCA], [https://pdbe.org/1m9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9n RCSB], [https://www.ebi.ac.uk/pdbsum/1m9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PUR9_CHICK PUR9_CHICK] Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.<ref>PMID:12501179</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m9/1m9n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m9n ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1M9N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with K, AMZ and XMP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M9N OCA].
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*[[Bifunctional purine biosynthesis protein PURH|Bifunctional purine biosynthesis protein PURH]]
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== References ==
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==Reference==
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<references/>
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Structural insights into the avian AICAR transformylase mechanism., Wolan DW, Greasley SE, Beardsley GP, Wilson IA, Biochemistry. 2002 Dec 31;41(52):15505-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12501179 12501179]
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__TOC__
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Beardsley, G.P.]]
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[[Category: Beardsley GP]]
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[[Category: Greasly, S.E.]]
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[[Category: Greasly SE]]
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[[Category: Wilson, I.A.]]
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[[Category: Wilson IA]]
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[[Category: Wolan, D.W.]]
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[[Category: Wolan DW]]
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[[Category: AMZ]]
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[[Category: K]]
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[[Category: XMP]]
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[[Category: 1 alpha + beta domain]]
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[[Category: 2 functional domains; impch domain = alpha/beta/alpha; aicar tfase = 2 alpha/beta/alpha domains]]
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[[Category: homodimer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:15:24 2007''
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Current revision

CRYSTAL STRUCTURE OF THE HOMODIMERIC BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME AVIAN ATIC IN COMPLEX WITH AICAR AND XMP AT 1.93 ANGSTROMS.

PDB ID 1m9n

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