1m9s

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(New page: 200px<br /><applet load="1m9s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m9s, resolution 2.65&Aring;" /> '''Crystal structure of...)
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[[Image:1m9s.jpg|left|200px]]<br /><applet load="1m9s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m9s, resolution 2.65&Aring;" />
 
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'''Crystal structure of Internalin B (InlB), a Listeria monocytogenes virulence protein containing SH3-like domains.'''<br />
 
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==Overview==
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==Crystal structure of Internalin B (InlB), a Listeria monocytogenes virulence protein containing SH3-like domains.==
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InlB, a surface-localized protein of Listeria monocytogenes, induces, phagocytosis in non-phagocytic mammalian cells by activating Met, a, receptor tyrosine kinase. InlB also binds glycosaminoglycans and the, protein gC1q-R, two additional host ligands implicated in invasion. We, present the structure of InlB, revealing a highly elongated molecule with, leucine-rich repeats that bind Met at one end, and GW domains that, dissociably bind the bacterial surface at the other. Surprisingly, the GW, domains are seen to resemble SH3 domains. Despite this, GW domains are, unlikely to act as functional mimics of SH3 domains since their potential, proline-binding sites are blocked or destroyed. However, we do show that, the GW domains, in addition to binding glycosaminoglycans, bind gC1q-R, specifically, and that this binding requires release of InlB from the, bacterial surface. Dissociable attachment to the bacterial surface via the, GW domains may be responsible for restricting Met activation to a small, localized area of the host cell and for coupling InlB-induced host, membrane dynamics with bacterial proximity during invasion.
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<StructureSection load='1m9s' size='340' side='right'caption='[[1m9s]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1m9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9S FirstGlance]. <br>
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1M9S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes] with TB and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M9S OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9s OCA], [https://pdbe.org/1m9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9s RCSB], [https://www.ebi.ac.uk/pdbsum/1m9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9s ProSAT]</span></td></tr>
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GW domains of the Listeria monocytogenes invasion protein InlB are SH3-like and mediate binding to host ligands., Marino M, Banerjee M, Jonquieres R, Cossart P, Ghosh P, EMBO J. 2002 Nov 1;21(21):5623-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12411480 12411480]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/INLB_LISMO INLB_LISMO] Mediates the entry of Listeria monocytogenes into cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m9/1m9s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m9s ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Listeria monocytogenes]]
[[Category: Listeria monocytogenes]]
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[[Category: Single protein]]
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[[Category: Banerjee M]]
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[[Category: Banerjee, M.]]
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[[Category: Cossart P]]
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[[Category: Cossart, P.]]
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[[Category: Ghosh P]]
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[[Category: Ghosh, P.]]
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[[Category: Jonquieres R]]
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[[Category: Jonquieres, R.]]
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[[Category: Marino M]]
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[[Category: Marino, M.]]
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[[Category: SO4]]
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[[Category: TB]]
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[[Category: cell invasion]]
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[[Category: gw domains]]
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[[Category: internalin]]
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[[Category: sh3 domains]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:15:34 2007''
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Current revision

Crystal structure of Internalin B (InlB), a Listeria monocytogenes virulence protein containing SH3-like domains.

PDB ID 1m9s

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