2ocy

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{{Seed}}
 
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[[Image:2ocy.png|left|200px]]
 
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==Complex of the guanine exchange factor Sec2p and the Rab GTPase Sec4p==
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The line below this paragraph, containing "STRUCTURE_2ocy", creates the "Structure Box" on the page.
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<StructureSection load='2ocy' size='340' side='right'caption='[[2ocy]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ocy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OCY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2ocy| PDB=2ocy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ocy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ocy OCA], [https://pdbe.org/2ocy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ocy RCSB], [https://www.ebi.ac.uk/pdbsum/2ocy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ocy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SEC2_YEAST SEC2_YEAST] Guanine nucleotide exchange factor for SEC4, catalyzing the dissociation of GDP from SEC4 and also potently promoting binding of GTP. Activation of SEC4 by SEC2 is needed for the directed transport of vesicles to sites of exocytosis. Binds the Rab GTPase YPT32, but does not have exhange activity on YPT32.<ref>PMID:9199166</ref> <ref>PMID:10747090</ref> <ref>PMID:12045183</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oc/2ocy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ocy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Rab GTPases, the largest subgroup in the superfamily of Ras-like GTPases, play regulatory roles in multiple steps of intracellular vesicle trafficking. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the interconversion of the GDP-bound, or inactive, form of Rab to the GTP-bound, or active, form. Relatively little is known of the mechanisms by which GEFs activate Rabs. Here, we present the crystal structure of the GEF domain of Sec2p in complex with its Rab partner Sec4p. The Sec2p GEF domain is a 220 Angstroms long coiled coil, striking in its simplicity and in the use of the coiled-coil motif for catalysis. The structure suggests a mechanism whereby Sec2p induces extensive structural rearrangements in the Sec4p switch regions and phosphate-binding loop that are incompatible with nucleotide binding. We show that Sec2p is specific for Sec4p and that specificity determinants reside in the two switch regions of Sec4p.
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===Complex of the guanine exchange factor Sec2p and the Rab GTPase Sec4p===
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A catalytic coiled coil: structural insights into the activation of the Rab GTPase Sec4p by Sec2p.,Dong G, Medkova M, Novick P, Reinisch KM Mol Cell. 2007 Feb 9;25(3):455-62. PMID:17289591<ref>PMID:17289591</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17289591}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2ocy" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17289591 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17289591}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2OCY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OCY OCA].
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==Reference==
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A catalytic coiled coil: structural insights into the activation of the Rab GTPase Sec4p by Sec2p., Dong G, Medkova M, Novick P, Reinisch KM, Mol Cell. 2007 Feb 9;25(3):455-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17289591 17289591]
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[[Category: Protein complex]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Dong, G.]]
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[[Category: Dong G]]
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[[Category: Reinisch, K M.]]
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[[Category: Reinisch KM]]
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[[Category: Coiled-coil]]
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[[Category: Gef]]
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[[Category: Guanine exchange factor]]
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[[Category: Rab]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:57:18 2008''
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Current revision

Complex of the guanine exchange factor Sec2p and the Rab GTPase Sec4p

PDB ID 2ocy

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