1mfz

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(New page: 200px<br /><applet load="1mfz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mfz, resolution 2.8&Aring;" /> '''Partially refined 2.8...)
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[[Image:1mfz.gif|left|200px]]<br /><applet load="1mfz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mfz, resolution 2.8&Aring;" />
 
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'''Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa'''<br />
 
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==Overview==
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==Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa==
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The enzyme GMD from Pseudomonas aeruginosa catalyzes the committed step in, the synthesis of the exopolysaccharide alginate. Alginate is a major, component of P. aeruginosa biofilms that protect the bacteria from the, host immune response and antibiotic therapy. The 1.55 A crystal structure, of GMD in ternary complex with its cofactor NAD(H) and product, GDP-mannuronic acid reveals that the enzyme forms a domain-swapped dimer, with two polypeptide chains contributing to each active site. The, extensive dimer interface provides multiple opportunities for intersubunit, communication. Comparison of the GMD structure with that of UDP-glucose, dehydrogenase reveals the structural basis of sugar binding specificity, that distinguishes these two related enzyme families. The high-resolution, structure of GMD provides detailed information on the active site of the, enzyme and a template for structure-based inhibitor design.
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<StructureSection load='1mfz' size='340' side='right'caption='[[1mfz]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mfz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MFZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDX:GUANOSINE+5-(TRIHYDROGEN+DIPHOSPHATE),+P-D-MANNOPYRANOSYL+ESTER'>GDX</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mfz OCA], [https://pdbe.org/1mfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mfz RCSB], [https://www.ebi.ac.uk/pdbsum/1mfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mfz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALGD_PSEAE ALGD_PSEAE] Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mf/1mfz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mfz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzyme GMD from Pseudomonas aeruginosa catalyzes the committed step in the synthesis of the exopolysaccharide alginate. Alginate is a major component of P. aeruginosa biofilms that protect the bacteria from the host immune response and antibiotic therapy. The 1.55 A crystal structure of GMD in ternary complex with its cofactor NAD(H) and product GDP-mannuronic acid reveals that the enzyme forms a domain-swapped dimer with two polypeptide chains contributing to each active site. The extensive dimer interface provides multiple opportunities for intersubunit communication. Comparison of the GMD structure with that of UDP-glucose dehydrogenase reveals the structural basis of sugar binding specificity that distinguishes these two related enzyme families. The high-resolution structure of GMD provides detailed information on the active site of the enzyme and a template for structure-based inhibitor design.
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==About this Structure==
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Crystal structure of GDP-mannose dehydrogenase: a key enzyme of alginate biosynthesis in P. aeruginosa.,Snook CF, Tipton PA, Beamer LJ Biochemistry. 2003 Apr 29;42(16):4658-68. PMID:12705829<ref>PMID:12705829</ref>
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1MFZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with GDX as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/GDP-mannose_6-dehydrogenase GDP-mannose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.132 1.1.1.132] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MFZ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of GDP-mannose dehydrogenase: a key enzyme of alginate biosynthesis in P. aeruginosa., Snook CF, Tipton PA, Beamer LJ, Biochemistry. 2003 Apr 29;42(16):4658-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12705829 12705829]
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</div>
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[[Category: GDP-mannose 6-dehydrogenase]]
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<div class="pdbe-citations 1mfz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Beamer LJ]]
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[[Category: Beamer, L.J.]]
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[[Category: Snook CF]]
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[[Category: Snook, C.F.]]
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[[Category: Tipton PA]]
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[[Category: Tipton, P.A.]]
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[[Category: GDX]]
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[[Category: domain-swapped dimer]]
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[[Category: enzyme complex with cofactor and product]]
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[[Category: rossman fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:23:24 2007''
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Current revision

Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa

PDB ID 1mfz

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