3bsq

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{{Seed}}
 
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[[Image:3bsq.png|left|200px]]
 
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==Crystal structure of human kallikrein 7 produced as a secretion protein in E.coli==
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The line below this paragraph, containing "STRUCTURE_3bsq", creates the "Structure Box" on the page.
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<StructureSection load='3bsq' size='340' side='right'caption='[[3bsq]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3bsq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3bsq| PDB=3bsq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsq OCA], [https://pdbe.org/3bsq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsq RCSB], [https://www.ebi.ac.uk/pdbsum/3bsq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KLK7_HUMAN KLK7_HUMAN] May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. SCCE cleaves insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bsq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bsq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human kallikrein 7, a major protease of human skin, has been synthesized directly in its native conformation in Escherichia coli by forcing the secretion of the newly synthesized polypeptide into the bacterial periplasm. The procedure yields a stable kallikrein 7 with highly specific activity that is inhibited efficiently by its specific inhibitor LEKTI domain 6. The protein was crystallized, and its three-dimensional structure was solved in the absence of protease inhibitors. The structure obtained agrees with that reported recently for human tissue kallikrein 7 crystallized in the presence of protease inhibitors from a preparation obtained in a baculovirus protein expression system. A model of the interaction between the protease and its inhibitor is proposed on the basis of both the three-dimensional structure of human tissue kallikrein 7 reported here and that of the LEKTI domain 6 solved previously by NMR.
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===Crystal structure of human kallikrein 7 produced as a secretion protein in E.coli===
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Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6).,Fernandez IS, Standker L, Magert HJ, Forssmann WG, Gimenez-Gallego G, Romero A J Mol Biol. 2008 Apr 11;377(5):1488-97. Epub 2008 Feb 9. PMID:18329042<ref>PMID:18329042</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3bsq" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18329042}}, adds the Publication Abstract to the page
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*[[Kallikrein 3D structures|Kallikrein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18329042 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18329042}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3BSQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSQ OCA].
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==Reference==
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Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6)., Fernandez IS, Standker L, Magert HJ, Forssmann WG, Gimenez-Gallego G, Romero A, J Mol Biol. 2008 Apr 11;377(5):1488-97. Epub 2008 Feb 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18329042 18329042]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Stratum corneum chymotryptic enzyme]]
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[[Category: Fernandez IS]]
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[[Category: Fernandez, I S.]]
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[[Category: Forssmann WG]]
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[[Category: Forssmann, W G.]]
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[[Category: Gimenez-Gallego G]]
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[[Category: Gimenez-Gallego, G.]]
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[[Category: Magert HJ]]
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[[Category: Magert, H J.]]
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[[Category: Romero A]]
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[[Category: Romero, A.]]
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[[Category: Standker L]]
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[[Category: Standker, L.]]
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[[Category: Alternative splicing]]
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[[Category: Glycoprotein]]
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[[Category: Hydrolase]]
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[[Category: Kallikrein]]
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[[Category: Ld6]]
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[[Category: Secreted]]
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[[Category: Serine protease]]
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[[Category: X-ray crystal structure]]
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[[Category: Zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:55:17 2008''
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Current revision

Crystal structure of human kallikrein 7 produced as a secretion protein in E.coli

PDB ID 3bsq

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