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1mgy

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(New page: 200px<br /><applet load="1mgy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mgy, resolution 2.0&Aring;" /> '''Structure of the D85S...)
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[[Image:1mgy.gif|left|200px]]<br /><applet load="1mgy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mgy, resolution 2.0&Aring;" />
 
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'''Structure of the D85S mutant of bacteriorhodopsin with bromide bound'''<br />
 
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==Overview==
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==Structure of the D85S mutant of bacteriorhodopsin with bromide bound==
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We report the crystal structure of a bromide-bound form of the D85S mutant, of bacteriorhodopsin, bR(D85S), a protein that uses light energy rather, than ATP to pump halide ions across the cell membrane. Comparison of the, structure of the halide-bound and halide-free states reveals that both, displacements of individual side-chain positions and concerted helical, movements occur on the extracellular side of the protein. Analysis of, these structural changes reveals how this ion pump first facilitates ion, uptake deep within the cell membrane and then prevents the backward escape, of ions later in the pumping cycle. Together with the information provided, by structures of intermediate states in the bacteriorhodopsin photocycle, this study also suggests the overall design principles that are necessary, for ion pumping.
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<StructureSection load='1mgy' size='340' side='right'caption='[[1mgy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MGY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mgy OCA], [https://pdbe.org/1mgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mgy RCSB], [https://www.ebi.ac.uk/pdbsum/1mgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mgy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mg/1mgy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mgy ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MGY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum] with BR, K, RET and LI1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MGY OCA].
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*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the bromide-bound D85S mutant of bacteriorhodopsin: principles of ion pumping., Facciotti MT, Cheung VS, Nguyen D, Rouhani S, Glaeser RM, Biophys J. 2003 Jul;85(1):451-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12829500 12829500]
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[[Category: Halobacterium salinarum]]
[[Category: Halobacterium salinarum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cheung, V.S.]]
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[[Category: Cheung VS]]
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[[Category: Facciotti, M.T.]]
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[[Category: Facciotti MT]]
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[[Category: Glaeser, R.M.]]
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[[Category: Glaeser RM]]
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[[Category: Nguyen, D.]]
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[[Category: Nguyen D]]
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[[Category: Rouhani, S.]]
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[[Category: Rouhani S]]
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[[Category: BR]]
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[[Category: K]]
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[[Category: LI1]]
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[[Category: RET]]
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[[Category: anion]]
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[[Category: bacteriorhodopsin]]
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[[Category: bromide]]
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[[Category: halide]]
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[[Category: pump]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:24:37 2007''
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Current revision

Structure of the D85S mutant of bacteriorhodopsin with bromide bound

PDB ID 1mgy

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