1x3u

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{{Seed}}
 
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[[Image:1x3u.png|left|200px]]
 
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==Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot==
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The line below this paragraph, containing "STRUCTURE_1x3u", creates the "Structure Box" on the page.
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<StructureSection load='1x3u' size='340' side='right'caption='[[1x3u]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1x3u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X3U FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x3u OCA], [https://pdbe.org/1x3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x3u RCSB], [https://www.ebi.ac.uk/pdbsum/1x3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x3u ProSAT]</span></td></tr>
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{{STRUCTURE_1x3u| PDB=1x3u | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIXJ_RHIME FIXJ_RHIME] FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x3/1x3u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x3u ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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FixJ is a response regulator of the two-component signal transduction pathway involved in the transcriptional activation of nitrogen fixation genes of Sinorhizobium meliloti. Upon phosphorylation, FixJ transcriptionally activates the fixK and nifA promoters. We identified a FixJ recognition sequence of 16 bp in the high affinity binding site of the fixK promoter by means of a gel shift assay. In addition, the solution structure of the truncated C-terminal DNA binding domain of FixJ (FixJC) was solved by NMR spectroscopy. FixJC contains five alpha-helices that encode a typical helix-turn-helix motif as a potential DNA binding core with the highest structural similarity toward the C-terminal DNA binding domain of NarL. The addition of the DNA fragment containing the recognition sequence of the high affinity FixJ binding site resulted in intermediate to slow exchange interactions on the NMR time scale in the spectrum of FixJC, while the exchange was rapid in the case of control DNA. These spectral data suggest that more than one molecule of FixJC binds to the recognition sequence, although FixJC alone is present in monomeric form in solution. This result is consistent with a scenario in which a transcriptionally active species of FixJ is a homodimer of the phosphorylated form.
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===Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot===
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Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium meliloti and its recognition of the fixK promoter.,Kurashima-Ito K, Kasai Y, Hosono K, Tamura K, Oue S, Isogai M, Ito Y, Nakamura H, Shiro Y Biochemistry. 2005 Nov 15;44(45):14835-44. PMID:16274231<ref>PMID:16274231</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16274231}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1x3u" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16274231 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16274231}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1X3U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X3U OCA].
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==Reference==
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Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium meliloti and its recognition of the fixK promoter., Kurashima-Ito K, Kasai Y, Hosono K, Tamura K, Oue S, Isogai M, Ito Y, Nakamura H, Shiro Y, Biochemistry. 2005 Nov 15;44(45):14835-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16274231 16274231]
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[[Category: Single protein]]
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[[Category: Sinorhizobium meliloti]]
[[Category: Sinorhizobium meliloti]]
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[[Category: Hosono, K.]]
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[[Category: Hosono K]]
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[[Category: Isogai, M.]]
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[[Category: Isogai M]]
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[[Category: Ito, Y.]]
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[[Category: Ito Y]]
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[[Category: Kasai, Y.]]
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[[Category: Kasai Y]]
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[[Category: Kurashima-Ito, K.]]
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[[Category: Kurashima-Ito K]]
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[[Category: Nakamura, H.]]
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[[Category: Nakamura H]]
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[[Category: Oue, S.]]
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[[Category: Oue S]]
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[[Category: Shiro, Y.]]
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[[Category: Shiro Y]]
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[[Category: Tamura, K.]]
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[[Category: Tamura K]]
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[[Category: Helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:08:10 2008''
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Current revision

Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot

PDB ID 1x3u

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