1miw

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(New page: 200px<br /><applet load="1miw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1miw, resolution 3.00&Aring;" /> '''Crystal structure of...)
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[[Image:1miw.gif|left|200px]]<br /><applet load="1miw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1miw, resolution 3.00&Aring;" />
 
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'''Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP'''<br />
 
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==Overview==
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==Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP==
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CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs, without using a nucleic acid template. The 3.0 A resolution crystal, structures of the CCA-adding enzyme from Bacillus stearothermophilus and, its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting, of four domains: head, neck, body, and tail. The head is structurally, homologous to the palm domain of DNA polymerase beta but has additional, structural features and functions. The neck, body, and tail represent new, protein folding motifs. The neck provides a specific template for the, incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit, has one active site capable of switching its base specificity between ATP, and CTP, an important component of the CCA-adding mechanism.
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<StructureSection load='1miw' size='340' side='right'caption='[[1miw]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1miw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MIW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1miw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1miw OCA], [https://pdbe.org/1miw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1miw RCSB], [https://www.ebi.ac.uk/pdbsum/1miw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1miw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CCA_GEOSE CCA_GEOSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1miw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1miw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template. The 3.0 A resolution crystal structures of the CCA-adding enzyme from Bacillus stearothermophilus and its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting of four domains: head, neck, body, and tail. The head is structurally homologous to the palm domain of DNA polymerase beta but has additional structural features and functions. The neck, body, and tail represent new protein folding motifs. The neck provides a specific template for the incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit has one active site capable of switching its base specificity between ATP and CTP, an important component of the CCA-adding mechanism.
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==About this Structure==
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Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP.,Li F, Xiong Y, Wang J, Cho HD, Tomita K, Weiner AM, Steitz TA Cell. 2002 Dec 13;111(6):815-24. PMID:12526808<ref>PMID:12526808</ref>
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1MIW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with MG and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MIW OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP., Li F, Xiong Y, Wang J, Cho HD, Tomita K, Weiner AM, Steitz TA, Cell. 2002 Dec 13;111(6):815-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12526808 12526808]
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</div>
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[[Category: Geobacillus stearothermophilus]]
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<div class="pdbe-citations 1miw" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Cho, H.D.]]
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[[Category: Li, F.]]
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[[Category: Steitz, T.A.]]
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[[Category: Wang, J.]]
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[[Category: Weiner, A.M.]]
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[[Category: Xiong, Y.]]
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[[Category: ATP]]
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[[Category: MG]]
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[[Category: cca-adding enzyme]]
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[[Category: trna nucleotidyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:26:36 2007''
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==See Also==
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*[[CCA-adding enzyme 3D structures|CCA-adding enzyme 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Geobacillus stearothermophilus]]
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[[Category: Large Structures]]
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[[Category: Cho HD]]
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[[Category: Li F]]
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[[Category: Steitz TA]]
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[[Category: Wang J]]
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[[Category: Weiner AM]]
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[[Category: Xiong Y]]

Current revision

Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP

PDB ID 1miw

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