1w4z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:15, 13 December 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1w4z.png|left|200px]]
 
-
<!--
+
==Structure of actinorhodin polyketide (actIII) Reductase==
-
The line below this paragraph, containing "STRUCTURE_1w4z", creates the "Structure Box" on the page.
+
<StructureSection load='1w4z' size='340' side='right'caption='[[1w4z]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1w4z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W4Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W4Z FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-
{{STRUCTURE_1w4z| PDB=1w4z | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w4z OCA], [https://pdbe.org/1w4z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w4z RCSB], [https://www.ebi.ac.uk/pdbsum/1w4z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w4z ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ACT3_STRCO ACT3_STRCO]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w4/1w4z_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w4z ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We have determined the 2.5 angstroms crystal structure of an active, tetrameric Streptomyces coelicolor type II polyketide ketoreductase (actIII) with its bound cofactor, NADP+. This structure shows a Rossman dinucleotide binding fold characteristic of SDR enzymes. Of two subunits in the crystallographic asymmetric unit, one is closed around the active site. Formate is observed in the open subunit, indicating possible carbonyl binding sites of the polyketide intermediate. Unlike previous models we observe crystal contacts that may mimic the KR-ACP interactions that may drive active site opening. Based on these observations, we have constructed a model for ACP and polyketide binding. We propose that binding of ACP triggers a conformational change from the closed to the open, active form of the enzyme. The polyketide chain enters the active site and reduction occurs. The model also suggests a general mechanism for ACP recognition which is applicable to a range of protein families.
-
===STRUCTURE OF ACTINORHODIN POLYKETIDE (ACTIII) REDUCTASE===
+
The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding.,Hadfield AT, Limpkin C, Teartasin W, Simpson TJ, Crosby J, Crump MP Structure. 2004 Oct;12(10):1865-75. PMID:15458634<ref>PMID:15458634</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_15458634}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1w4z" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 15458634 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_15458634}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
1W4Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W4Z OCA].
+
-
 
+
-
==Reference==
+
-
The crystal structure of the actIII actinorhodin polyketide reductase: proposed mechanism for ACP and polyketide binding., Hadfield AT, Limpkin C, Teartasin W, Simpson TJ, Crosby J, Crump MP, Structure. 2004 Oct;12(10):1865-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15458634 15458634]
+
-
[[Category: Single protein]]
+
[[Category: Streptomyces coelicolor]]
[[Category: Streptomyces coelicolor]]
-
[[Category: Crosby, J.]]
+
[[Category: Crosby J]]
-
[[Category: Crump, M P.]]
+
[[Category: Crump MP]]
-
[[Category: Hadfield, A T.]]
+
[[Category: Hadfield AT]]
-
[[Category: Limpkin, C.]]
+
[[Category: Limpkin C]]
-
[[Category: Simpson, T J.]]
+
[[Category: Simpson TJ]]
-
[[Category: Teartasin, W.]]
+
[[Category: Teartasin W]]
-
[[Category: Acp binding]]
+
-
[[Category: Antibiotic biosynthesis]]
+
-
[[Category: Ketoreductase]]
+
-
[[Category: Sdr]]
+
-
[[Category: Type ii polyketide synthesis]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:38:25 2008''
+

Current revision

Structure of actinorhodin polyketide (actIII) Reductase

PDB ID 1w4z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w4z"
Personal tools