1mli

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(New page: 200px<br /><applet load="1mli" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mli, resolution 3.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1mli.gif|left|200px]]<br /><applet load="1mli" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mli, resolution 3.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION==
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The crystal structure of muconolactone isomerase from Pseudomonas putida, a unique molecule with ten 96 amino acid subunits and 5-fold, and 2-fold, symmetries, has been solved at 3.3 A resolution. The non-crystallographic, symmetries were used to refine the initial single isomorphous replacement, phases and produce an interpretable 10-fold averaged map. The backbone, trace is complete and confirmed by the amino acid sequence fit. Each, subunit is composed of a body with two alpha-helices and an antiparallel, twisted beta-sheet of four strands, and an extended arm. The helices and, the sheet fold to form a two-layered structure with an enclosed, hydrophobic core and a partially formed putative active site pocket. The, C-terminal arm of another subunit related by a local dyad symmetry extends, over the core to complete this pocket. The decameric protein is almost, spherical, with the helices forming the external coat. There is a large, hydrophilic cavity in the center with open ends along the 5-fold axis., Molecular interactions between subunits are extensive. Each subunit, contacts four neighbors and loses nearly 40% of its solvent contact area, on oligomerization.
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<StructureSection load='1mli' size='340' side='right'caption='[[1mli]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mli]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLI FirstGlance]. <br>
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1MLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Muconolactone_Delta-isomerase Muconolactone Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.4 5.3.3.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MLI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mli OCA], [https://pdbe.org/1mli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mli RCSB], [https://www.ebi.ac.uk/pdbsum/1mli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mli ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of muconolactone isomerase at 3.3 A resolution., Katti SK, Katz BA, Wyckoff HW, J Mol Biol. 1989 Feb 5;205(3):557-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2926818 2926818]
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== Function ==
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[[Category: Muconolactone Delta-isomerase]]
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[https://www.uniprot.org/uniprot/CATC_PSEPU CATC_PSEPU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ml/1mli_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mli ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: Single protein]]
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[[Category: Katti SK]]
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[[Category: Katti, S.K.]]
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[[Category: Katz BA]]
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[[Category: Katz, B.A.]]
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[[Category: Wyckoff HW]]
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[[Category: Wyckoff, H.W.]]
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[[Category: intramolecular oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:30:22 2007''
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Current revision

CRYSTAL STRUCTURE OF MUCONOLACTONE ISOMERASE AT 3.3 ANGSTROMS RESOLUTION

PDB ID 1mli

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