1mnn

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(New page: 200px<br /><applet load="1mnn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mnn, resolution 1.4&Aring;" /> '''Structure of the spor...)
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[[Image:1mnn.gif|left|200px]]<br /><applet load="1mnn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mnn, resolution 1.4&Aring;" />
 
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'''Structure of the sporulation specific transcription factor Ndt80 bound to DNA'''<br />
 
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==Overview==
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==Structure of the sporulation specific transcription factor Ndt80 bound to DNA==
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Progression through the middle phase of sporulation in Saccharomyces, cerevisiae is promoted by the successful completion of recombination at, the end of prophase I. Completion of meiotic recombination allows the, activation of the sporulation-specific transcription factor Ndt80, which, binds to a specific DNA sequence, the middle sporulation element (MSE), and activates approximately 150 genes to enable progression through, meiosis. Here, we isolate the DNA-binding domain of Ndt80 and determine, its crystal structure both free and in complex with an MSE-containing DNA., The structure reveals that Ndt80 is a member of the Ig-fold family of, transcription factors. The structure of the DNA-bound form, refined at 1.4, A, reveals an unexpected mode of recognition of 5'-pyrimidine- guanine-3', dinucleotide steps by arginine residues that simultaneously recognize the, 3'-guanine base through hydrogen bond interactions and the 5'-pyrimidine, through stacking/van der Waals interactions. Analysis of the DNA-binding, affinities of MSE mutants demonstrates the central importance of these, interactions, and of the AT-rich portion of the MSE. Functional, similarities between Ndt80 and the Caenorhabditis elegans p53 homolog, suggest an evolutionary link between Ndt80 and the p53 family.
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<StructureSection load='1mnn' size='340' side='right'caption='[[1mnn]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mnn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MNN FirstGlance]. <br>
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1MNN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MNN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mnn OCA], [https://pdbe.org/1mnn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mnn RCSB], [https://www.ebi.ac.uk/pdbsum/1mnn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mnn ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of the sporulation-specific transcription factor Ndt80 bound to DNA., Lamoureux JS, Stuart D, Tsang R, Wu C, Glover JN, EMBO J. 2002 Nov 1;21(21):5721-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12411490 12411490]
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== Function ==
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[https://www.uniprot.org/uniprot/NDT80_YEAST NDT80_YEAST] Transcription factor required for successful completion of meiosis and spore formation. Gets activated after completion of meiotic recombination at the end of prophase I. Recognizes and binds to the middle sporulation element (MSE) 5'-C[AG]CAAA[AT]-3' in the promoter region of stage-specific genes that are required for progression through meiosis and sporulation. Competes for binding to MSE with the transcriptional repressor SUM1, which represses middle sporulation-specific genes during mitosis and early sporulation.<ref>PMID:8524222</ref> <ref>PMID:9660952</ref> <ref>PMID:9742092</ref> <ref>PMID:12832469</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/1mnn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mnn ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Glover JN]]
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[[Category: Glover, J.N.]]
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[[Category: Lamoureux JS]]
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[[Category: Lamoureux, J.S.]]
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[[Category: Stuart D]]
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[[Category: Stuart, D.]]
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[[Category: Tsang R]]
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[[Category: Tsang, R.]]
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[[Category: Wu C]]
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[[Category: Wu, C.]]
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[[Category: ig fold]]
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[[Category: protein_dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:33:40 2007''
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Current revision

Structure of the sporulation specific transcription factor Ndt80 bound to DNA

PDB ID 1mnn

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