2v6x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:59, 23 October 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2v6x.png|left|200px]]
 
-
<!--
+
==Stractural insight into the interaction between ESCRT-III and Vps4==
-
The line below this paragraph, containing "STRUCTURE_2v6x", creates the "Structure Box" on the page.
+
<StructureSection load='2v6x' size='340' side='right'caption='[[2v6x]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2v6x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V6X FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_2v6x| PDB=2v6x | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6x OCA], [https://pdbe.org/2v6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v6x RCSB], [https://www.ebi.ac.uk/pdbsum/2v6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v6x ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/VPS4_YEAST VPS4_YEAST] Involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Required for multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex.<ref>PMID:11329380</ref> <ref>PMID:9155008</ref> <ref>PMID:9606181</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v6/2v6x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v6x ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The AAA+ ATPases are essential for various activities such as membrane trafficking, organelle biogenesis, DNA replication, intracellular locomotion, cytoskeletal remodelling, protein folding and proteolysis. The AAA ATPase Vps4, which is central to endosomal traffic to lysosomes, retroviral budding and cytokinesis, dissociates ESCRT complexes (the endosomal sorting complexes required for transport) from membranes. Here we show that, of the six ESCRT--related subunits in yeast, only Vps2 and Did2 bind the MIT (microtubule interacting and transport) domain of Vps4, and that the carboxy-terminal 30 residues of the subunits are both necessary and sufficient for interaction. We determined the crystal structure of the Vps2 C terminus in a complex with the Vps4 MIT domain, explaining the basis for selective ESCRT-III recognition. MIT helices alpha2 and alpha3 recognize a (D/E)xxLxxRLxxL(K/R) motif, and mutations within this motif cause sorting defects in yeast. Our crystal structure of the amino-terminal domain of an archaeal AAA ATPase of unknown function shows that it is closely related to the MIT domain of Vps4. The archaeal ATPase interacts with an archaeal ESCRT-III-like protein even though these organisms have no endomembrane system, suggesting that the Vps4/ESCRT-III partnership is a relic of a function that pre-dates the divergence of eukaryotes and Archaea.
-
===STRACTURAL INSIGHT INTO THE INTERACTION BETWEEN ESCRT-III AND VPS4===
+
Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4.,Obita T, Saksena S, Ghazi-Tabatabai S, Gill DJ, Perisic O, Emr SD, Williams RL Nature. 2007 Oct 11;449(7163):735-9. PMID:17928861<ref>PMID:17928861</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2v6x" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_17928861}}, adds the Publication Abstract to the page
+
*[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 17928861 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_17928861}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2V6X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6X OCA].
+
-
 
+
-
==Reference==
+
-
Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4., Obita T, Saksena S, Ghazi-Tabatabai S, Gill DJ, Perisic O, Emr SD, Williams RL, Nature. 2007 Oct 11;449(7163):735-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17928861 17928861]
+
-
[[Category: Protein complex]]
+
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Vesicle-fusing ATPase]]
+
[[Category: Emr SD]]
-
[[Category: Emr, S D.]]
+
[[Category: Ghazi-Tabatabai S]]
-
[[Category: Ghazi-Tabatabai, S.]]
+
[[Category: Obita T]]
-
[[Category: Obita, T.]]
+
[[Category: Perisic O]]
-
[[Category: Perisic, O.]]
+
[[Category: Saksena S]]
-
[[Category: Saksena, S.]]
+
[[Category: Williams RL]]
-
[[Category: Williams, R L.]]
+
-
[[Category: Aaa-atpase]]
+
-
[[Category: Atp-binding]]
+
-
[[Category: Chmp2a]]
+
-
[[Category: Chmp2b]]
+
-
[[Category: Coiled coil]]
+
-
[[Category: Cytoplasm]]
+
-
[[Category: Endosome]]
+
-
[[Category: Escrt-iii]]
+
-
[[Category: Multivesicular]]
+
-
[[Category: Mvb]]
+
-
[[Category: Nucleotide-binding]]
+
-
[[Category: Protein transport]]
+
-
[[Category: Skd1]]
+
-
[[Category: Transport]]
+
-
[[Category: Vacuolar protein sorting]]
+
-
[[Category: Vacuole]]
+
-
[[Category: Vps2]]
+
-
[[Category: Vps4]]
+
-
[[Category: Vps4a]]
+
-
[[Category: Vps4b]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:16:09 2008''
+

Current revision

Stractural insight into the interaction between ESCRT-III and Vps4

PDB ID 2v6x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v6x"
Personal tools