1mol

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(New page: 200px<br /><applet load="1mol" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mol, resolution 1.7&Aring;" /> '''TWO CRYSTAL STRUCTURE...)
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[[Image:1mol.gif|left|200px]]<br /><applet load="1mol" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mol, resolution 1.7&Aring;" />
 
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'''TWO CRYSTAL STRUCTURES OF A POTENTLY SWEET PROTEIN: NATURAL MONELLIN AT 2.75 ANGSTROMS RESOLUTION AND SINGLE-CHAIN MONELLIN AT 1.7 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==TWO CRYSTAL STRUCTURES OF A POTENTLY SWEET PROTEIN: NATURAL MONELLIN AT 2.75 ANGSTROMS RESOLUTION AND SINGLE-CHAIN MONELLIN AT 1.7 ANGSTROMS RESOLUTION==
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Two refined structures of the sweet-tasting protein monellin are, presented. The structure of natural monellin has been refined at 2.75 A, resolution. The final model consists of four monellin molecules in the, asymmetric unit, encompassing 3136 non-hydrogen atoms. The, crystallographic R-factor is 0.193 for the 8853 reflections between 6.0 A, and 2.75 A resolution, and the root-mean-square deviations from ideality, are 0.017 A for bond lengths and 3.6 degrees for bond angles. The refined, structure generally confirms, with some difference in detail, the initial, backbone model of monellin that was based on 3.0 A resolution data., Single-chain monellin (scm) was genetically engineered by fusing the two, chains of monellin into a single 94-residue polypeptide. Using the refined, monellin coordinates as a search model, the crystal structure of scm has, been solved with the techniques of molecular replacement, and has been, refined against data to 1.7 A resolution. The final model consists of two, scm molecules per asymmetric unit, and includes 137 bound water molecules., The crystallographic R-factor for this model is 0.174 for the 15,053, reflections (magnitude of FO magnitude of &gt; 2 sigma (FO)) between 6.0 A, and 1.7 A resolution. The root-mean-square deviations from ideal bond, lengths and angles are 0.015 A and 2.86 degrees, respectively, and the, average coordinate error is approximately 0.2 A, as estimated from a, Luzzati plot. The error in the model was also estimated by comparing the, two molecules in the asymmetric unit. The most significant differences, between the two molecules occur in loop regions and at the C terminus of, the protein, and are generally correlated to differences in crystal, packing contacts. Linking the two chains of monellin has not substantially, altered the structure beyond the region immediately surrounding the new, peptide bond. Like natural monellin, the conformation of scm is dominated, by a 17-residue alpha-helix folded into the concave side of a twisted, five-strand anti-parallel beta-sheet. We expect that the availability of a, high-resolution structure of scm, along with the convenience of producing, site-specific mutants of this protein, will make scm a good model with, which to probe the structural basis of sweetness.
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<StructureSection load='1mol' size='340' side='right'caption='[[1mol]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mol]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOL FirstGlance]. <br>
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1MOL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MOL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mol OCA], [https://pdbe.org/1mol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mol RCSB], [https://www.ebi.ac.uk/pdbsum/1mol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mol ProSAT]</span></td></tr>
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==Reference==
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</table>
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Two crystal structures of a potently sweet protein. Natural monellin at 2.75 A resolution and single-chain monellin at 1.7 A resolution., Somoza JR, Jiang F, Tong L, Kang CH, Cho JM, Kim SH, J Mol Biol. 1993 Nov 20;234(2):390-404. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8230222 8230222]
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== Function ==
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[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.
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__TOC__
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</StructureSection>
[[Category: Dioscoreophyllum cumminsii]]
[[Category: Dioscoreophyllum cumminsii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kim, S.H.]]
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[[Category: Kim S-H]]
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[[Category: Somoza, J.R.]]
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[[Category: Somoza JR]]
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[[Category: sweet-tasting protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:35:16 2007''
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Current revision

TWO CRYSTAL STRUCTURES OF A POTENTLY SWEET PROTEIN: NATURAL MONELLIN AT 2.75 ANGSTROMS RESOLUTION AND SINGLE-CHAIN MONELLIN AT 1.7 ANGSTROMS RESOLUTION

PDB ID 1mol

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