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1mos

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(New page: 200px<br /><applet load="1mos" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mos, resolution 2.00&Aring;" /> '''ISOMERASE DOMAIN OF ...)
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[[Image:1mos.gif|left|200px]]<br /><applet load="1mos" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mos, resolution 2.00&Aring;" />
 
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'''ISOMERASE DOMAIN OF GLUCOSAMINE 6-PHOSPHATE SYNTHASE COMPLEXED WITH 2-AMINO-2-DEOXYGLUCITOL 6-PHOSPHATE'''<br />
 
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==Overview==
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==ISOMERASE DOMAIN OF GLUCOSAMINE 6-PHOSPHATE SYNTHASE COMPLEXED WITH 2-AMINO-2-DEOXYGLUCITOL 6-PHOSPHATE==
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Glucosamine 6-phosphate synthase converts fructose-6P into glucosamine-6P, or glucose-6P depending on the presence or absence of glutamine. The, isomerase activity is associated with a 40-kDa C-terminal domain, which, has already been characterized crystallographically. Now the, three-dimensional structures of the complexes with the reaction product, glucose-6P and with the transition state analog 2-amino-2-deoxyglucitol-6P, have been determined. Glucose-6P binds in a cyclic form whereas, 2-amino-2-deoxyglucitol-6P is in an extended conformation. The information, on ligand-protein interactions observed in the crystal structures together, with the isotope exchange and site-directed mutagenesis data allow us to, propose a mechanism of the isomerase activity of glucosamine-6P synthase., The sugar phosphate isomerization involves a ring opening step catalyzed, by His504 and an enolization step with Glu488 catalyzing the hydrogen, transfer from C1 to C2 of the substrate. The enediol intermediate is, stabilized by a helix dipole and the epsilon-amino group of Lys603. Lys485, may play a role in deprotonating the hydroxyl O1 of the intermediate.
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<StructureSection load='1mos' size='340' side='right'caption='[[1mos]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mos]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MOS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGP:2-DEOXY-2-AMINO+GLUCITOL-6-PHOSPHATE'>AGP</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mos OCA], [https://pdbe.org/1mos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mos RCSB], [https://www.ebi.ac.uk/pdbsum/1mos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mos ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLMS_ECOLI GLMS_ECOLI] Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mo/1mos_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mos ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glucosamine 6-phosphate synthase converts fructose-6P into glucosamine-6P or glucose-6P depending on the presence or absence of glutamine. The isomerase activity is associated with a 40-kDa C-terminal domain, which has already been characterized crystallographically. Now the three-dimensional structures of the complexes with the reaction product glucose-6P and with the transition state analog 2-amino-2-deoxyglucitol-6P have been determined. Glucose-6P binds in a cyclic form whereas 2-amino-2-deoxyglucitol-6P is in an extended conformation. The information on ligand-protein interactions observed in the crystal structures together with the isotope exchange and site-directed mutagenesis data allow us to propose a mechanism of the isomerase activity of glucosamine-6P synthase. The sugar phosphate isomerization involves a ring opening step catalyzed by His504 and an enolization step with Glu488 catalyzing the hydrogen transfer from C1 to C2 of the substrate. The enediol intermediate is stabilized by a helix dipole and the epsilon-amino group of Lys603. Lys485 may play a role in deprotonating the hydroxyl O1 of the intermediate.
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==About this Structure==
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The mechanism of sugar phosphate isomerization by glucosamine 6-phosphate synthase.,Teplyakov A, Obmolova G, Badet-Denisot MA, Badet B Protein Sci. 1999 Mar;8(3):596-602. PMID:10091662<ref>PMID:10091662</ref>
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1MOS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, NA, AGP and MES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamine--fructose-6-phosphate_transaminase_(isomerizing) Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.16 2.6.1.16] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MOS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The mechanism of sugar phosphate isomerization by glucosamine 6-phosphate synthase., Teplyakov A, Obmolova G, Badet-Denisot MA, Badet B, Protein Sci. 1999 Mar;8(3):596-602. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10091662 10091662]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1mos" style="background-color:#fffaf0;"></div>
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[[Category: Glutamine--fructose-6-phosphate transaminase (isomerizing)]]
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[[Category: Single protein]]
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[[Category: Badet, B.]]
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[[Category: Badet-Denisot, M.A.]]
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[[Category: Obmolova, G.]]
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[[Category: Teplyakov, A.]]
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[[Category: AGP]]
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[[Category: MES]]
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[[Category: NA]]
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[[Category: SO4]]
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[[Category: aminotransferase]]
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[[Category: glutamine amidotransferase]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:35:37 2007''
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==See Also==
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*[[Glucosamine 6-phosphate synthase|Glucosamine 6-phosphate synthase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Badet B]]
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[[Category: Badet-Denisot MA]]
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[[Category: Obmolova G]]
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[[Category: Teplyakov A]]

Current revision

ISOMERASE DOMAIN OF GLUCOSAMINE 6-PHOSPHATE SYNTHASE COMPLEXED WITH 2-AMINO-2-DEOXYGLUCITOL 6-PHOSPHATE

PDB ID 1mos

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