1k3i

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{{Seed}}
 
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[[Image:1k3i.png|left|200px]]
 
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==Crystal Structure of the Precursor of Galactose Oxidase==
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The line below this paragraph, containing "STRUCTURE_1k3i", creates the "Structure Box" on the page.
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<StructureSection load='1k3i' size='340' side='right'caption='[[1k3i]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1k3i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_sp. Fusarium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K3I FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
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{{STRUCTURE_1k3i| PDB=1k3i | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3i OCA], [https://pdbe.org/1k3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k3i RCSB], [https://www.ebi.ac.uk/pdbsum/1k3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k3i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAOA_GIBZA GAOA_GIBZA] Catalyzes the sterospecific oxidation of primary alcohols to the corresponding aldehydes. The biologically relevant substrate of the enzyme is not known as the enzyme exhibits broad substrate specificity from small alcohols through sugars to oligo- and polysaccharides.<ref>PMID:13641238</ref> <ref>PMID:4441089</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/1k3i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k3i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Galactose oxidase (EC ) is a monomeric enzyme that contains a single copper ion and catalyses the stereospecific oxidation of primary alcohols to their corresponding aldehydes. The protein contains an unusual covalent thioether bond between a tyrosine, which acts as a radical center during the two-electron reaction, and a cysteine. The enzyme is produced in a precursor form lacking the thioether bond and also possessing an additional 17-aa pro-sequence at the N terminus. Previous work has shown that the aerobic addition of Cu(2+) to the precursor is sufficient to generate fully processed mature enzyme. The structure of the precursor protein has been determined to 1.4 A, revealing the location of the pro-sequence and identifying structural differences between the precursor and the mature protein. Structural alignment of the precursor and mature forms of galactose oxidase shows that five regions of main chain and some key residues of the active site differ significantly between the two forms. The precursor structure provides a starting point for modeling the chemistry of thioether bond formation and pro-sequence cleavage.
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===Crystal Structure of the Precursor of Galactose Oxidase===
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Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme.,Firbank SJ, Rogers MS, Wilmot CM, Dooley DM, Halcrow MA, Knowles PF, McPherson MJ, Phillips SE Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):12932-7. PMID:11698678<ref>PMID:11698678</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1k3i" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11698678}}, adds the Publication Abstract to the page
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*[[Galactose oxidase|Galactose oxidase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11698678 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11698678}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Fusarium sp]]
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1K3I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sp. Fusarium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3I OCA].
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[[Category: Large Structures]]
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[[Category: Dooley DM]]
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==Reference==
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[[Category: Firbank SJ]]
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Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme., Firbank SJ, Rogers MS, Wilmot CM, Dooley DM, Halcrow MA, Knowles PF, McPherson MJ, Phillips SE, Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):12932-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11698678 11698678]
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[[Category: Halcrow MA]]
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[[Category: Fusarium sp.]]
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[[Category: Knowles PF]]
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[[Category: Galactose oxidase]]
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[[Category: McPherson MJ]]
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[[Category: Single protein]]
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[[Category: Phillips SEV]]
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[[Category: Dooley, D M.]]
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[[Category: Rogers MS]]
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[[Category: Firbank, S J.]]
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[[Category: Wilmot CM]]
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[[Category: Halcrow, M A.]]
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[[Category: Knowles, P F.]]
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[[Category: McPherson, M J.]]
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[[Category: Phillips, S E.V.]]
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[[Category: Rogers, M S.]]
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[[Category: Wilmot, C M.]]
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[[Category: 7 blade beta propeller]]
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[[Category: Precursor of copper enzyme.]]
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[[Category: Prosequence form]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:29:58 2008''
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Current revision

Crystal Structure of the Precursor of Galactose Oxidase

PDB ID 1k3i

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