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1mpj

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(New page: 200px<br /><applet load="1mpj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mpj, resolution 2.3&Aring;" /> '''X-RAY CRYSTALLOGRAPHI...)
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[[Image:1mpj.jpg|left|200px]]<br /><applet load="1mpj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mpj, resolution 2.3&Aring;" />
 
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'''X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL'''<br />
 
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==Overview==
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==X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL==
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Three X-ray crystallographic studies have been carried out on pig insulin, in the presence of three ligands, thiocyanate, methylparaben (methyl, p-hydroxybenzoate), and phenol. In each case, rhombohedral crystals were, obtained, which diffracted to 1.8, 1.9, and 2.3 A, respectively. Each, crystal structure was very similar to that of 4-zinc pig insulin, which, was used as a starting model for PROLSQ refinement (Collaborative, Computational Project, Number 4, 1994). The R factors for the refined, structures of thiocyanate insulin, methylparaben insulin, and phenol, insulin were 19.6, 18.4, and 19.1, respectively. Each crystal structure, consists of T3R3f insulin hexamers with two zinc ions per hexamer. In the, R3f trimer of the thiocyanate insulin hexamer, one thiocyanate ion is, coordinated to the zinc on the hexamer 3-fold axis, but there is no, evidence of zinc ion binding in the off-axis zinc ion sites seen in the, 4-zinc pig insulin structure. In the methylparaben insulin and phenol, insulin hexamers, the phenolic ligands are bound at the dimer-dimer, interfaces in the R3f trimers in a manner similar to that of phenol in R6, phenol insulin. The binding of methylparaben appears to make the hexamer, more compact by drawing the A and the B chains closer together in the, binding site. In all three structures presented herein, the conformations, of the first three residues of the B chain in the R3f trimer are extended, rather than alpha-helical, as is seen in R6 phenol insulin. The energetics, of ligand binding in the insulin hexamer are discussed.
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<StructureSection load='1mpj' size='340' side='right'caption='[[1mpj]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mpj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MPJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mpj OCA], [https://pdbe.org/1mpj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mpj RCSB], [https://www.ebi.ac.uk/pdbsum/1mpj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mpj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/INS_PIG INS_PIG] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mp/1mpj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mpj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MPJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with ZN, CL and IPH as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MPJ OCA].
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*[[Insulin 3D Structures|Insulin 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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X-ray crystallographic studies on hexameric insulins in the presence of helix-stabilizing agents, thiocyanate, methylparaben, and phenol., Whittingham JL, Chaudhuri S, Dodson EJ, Moody PC, Dodson GG, Biochemistry. 1995 Nov 28;34(47):15553-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7492558 7492558]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Dodson, E.J.]]
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[[Category: Dodson EJ]]
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[[Category: Dodson, G.G.]]
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[[Category: Dodson GG]]
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[[Category: Moody, P.C.E.]]
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[[Category: Moody PCE]]
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[[Category: Whittingham, J.L.]]
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[[Category: Whittingham JL]]
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[[Category: CL]]
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[[Category: IPH]]
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[[Category: ZN]]
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[[Category: hormone]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:36:42 2007''
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Current revision

X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL

PDB ID 1mpj

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