1p6r

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{{Seed}}
 
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[[Image:1p6r.png|left|200px]]
 
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==Solution structure of the DNA binding domain of the repressor BlaI.==
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The line below this paragraph, containing "STRUCTURE_1p6r", creates the "Structure Box" on the page.
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<StructureSection load='1p6r' size='340' side='right'caption='[[1p6r]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1p6r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P6R FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p6r OCA], [https://pdbe.org/1p6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p6r RCSB], [https://www.ebi.ac.uk/pdbsum/1p6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p6r ProSAT]</span></td></tr>
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{{STRUCTURE_1p6r| PDB=1p6r | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLAI_BACLI BLAI_BACLI] Transcriptional repressor that constitutively blocks expression of beta-lactamase. Regulates genes involved in antibiotic resistance. Binds DNA as a dimer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/1p6r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p6r ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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beta-Lactamase and penicillin-binding protein PBP2' mediate staphylococcal resistance to beta-lactam antibiotics, which are otherwise highly clinically effective. Two repressors (BlaI and MecI) regulate expression of these inducible proteins. Here, we present the first solution structure of the 82 amino acid residue DNA-binding domain of Bacillus licheniformis BlaI which is very similar in primary sequence to the medically significant Staphyloccocal BlaI and MecI proteins. This structure is composed of a compact core of three alpha-helices and a three-stranded beta-sheet typical of the winged helix protein (WHP) family. The protein/DNA complex was studied by NMR chemical shift comparison between the free and complexed forms of BlaI. Residues involved in DNA interaction were identified and a WHP canonical model of interaction with the operators is proposed. In this model, specific contacts occur between the base-pairs of the TACA motif and conserved amino acid residues of the repressor helix H3. These results help toward understanding the repression and induction mechanism of the genes coding for beta-lactamase and PBP2'.
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===Solution structure of the DNA binding domain of the repressor BlaI.===
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Solution structural study of BlaI: implications for the repression of genes involved in beta-lactam antibiotic resistance.,Melckebeke HV, Vreuls C, Gans P, Filee P, Llabres G, Joris B, Simorre JP J Mol Biol. 2003 Oct 31;333(4):711-20. PMID:14568532<ref>PMID:14568532</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14568532}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1p6r" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14568532 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14568532}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1P6R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6R OCA].
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==Reference==
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Solution structural study of BlaI: implications for the repression of genes involved in beta-lactam antibiotic resistance., Melckebeke HV, Vreuls C, Gans P, Filee P, Llabres G, Joris B, Simorre JP, J Mol Biol. 2003 Oct 31;333(4):711-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14568532 14568532]
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[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Filee, P.]]
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[[Category: Filee P]]
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[[Category: Gans, P.]]
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[[Category: Gans P]]
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[[Category: Joris, B.]]
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[[Category: Joris B]]
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[[Category: Llabres, G.]]
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[[Category: Llabres G]]
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[[Category: Melckebeke, H V.]]
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[[Category: Melckebeke HV]]
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[[Category: Simorre, J P.]]
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[[Category: Simorre JP]]
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[[Category: Vreuls, C.]]
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[[Category: Vreuls C]]
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[[Category: Bacterial resistance to antibiotic]]
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[[Category: Dna-binding]]
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[[Category: Repressor]]
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[[Category: Transcription regulation]]
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[[Category: Winged helix protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:49:19 2008''
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Current revision

Solution structure of the DNA binding domain of the repressor BlaI.

PDB ID 1p6r

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OCA

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