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1qlw

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(New page: 200px<br /> <applet load="1qlw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qlw, resolution 1.09&Aring;" /> '''THE ATOMIC RESOLUTI...)
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[[Image:1qlw.gif|left|200px]]<br />
 
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<applet load="1qlw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qlw, resolution 1.09&Aring;" />
 
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'''THE ATOMIC RESOLUTION STRUCTURE OF A NOVEL BACTERIAL ESTERASE'''<br />
 
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==Overview==
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==The Atomic Resolution Structure of a Novel Bacterial Esterase==
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Background: A novel bacterial esterase that cleaves esters on halogenated, cyclic compounds has been isolated from an Alcaligenes species. This, esterase 713 is encoded by a 1062 base pair gene. The presence of a leader, sequence of 27 amino acids suggests that this enzyme is exported from the, cytosol. Esterase 713 has been over-expressed in Agrobacterium without, this leader sequence. Its amino acid sequence shows no significant, homology to any known protein sequence. Results: The crystal structure of, esterase 713 has been determined by multiple isomorphous replacement and, refined to 1. 1 A resolution. The subunits of this dimeric enzyme comprise, a single domain with an alpha/beta hydrolase fold. The catalytic triad has, been identified as Ser206-His298-Glu230. The acidic residue of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10673440 (full description)]]
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<StructureSection load='1qlw' size='340' side='right'caption='[[1qlw]], [[Resolution|resolution]] 1.09&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qlw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_sp. Alcaligenes sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QLW FirstGlance]. <br>
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1QLW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Alcaligenes_sp. Alcaligenes sp.]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QLW OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.09&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qlw OCA], [https://pdbe.org/1qlw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qlw RCSB], [https://www.ebi.ac.uk/pdbsum/1qlw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qlw ProSAT]</span></td></tr>
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The atomic-resolution structure of a novel bacterial esterase., Bourne PC, Isupov MN, Littlechild JA, Structure. 2000 Feb 15;8(2):143-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10673440 10673440]
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</table>
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[[Category: Alcaligenes sp.]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q7SIA5_ALCSP Q7SIA5_ALCSP]
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[[Category: Bourne, P.C.]]
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== Evolutionary Conservation ==
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[[Category: Isupov, M.N.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Littlechild, J.A.]]
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Check<jmol>
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[[Category: SO4]]
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<jmolCheckbox>
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[[Category: alpha/beta hydrolase]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ql/1qlw_consurf.spt"</scriptWhenChecked>
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[[Category: anisotropic refinement]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: atomic resolution]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: esterase]]
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qlw ConSurf].
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:31:08 2007''
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Alcaligenes sp]]
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[[Category: Large Structures]]
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[[Category: Bourne PC]]
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[[Category: Isupov MN]]
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[[Category: Littlechild JA]]

Current revision

The Atomic Resolution Structure of a Novel Bacterial Esterase

PDB ID 1qlw

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