2pmu

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{{Seed}}
 
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[[Image:2pmu.png|left|200px]]
 
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==Crystal structure of the DNA-binding domain of PhoP==
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The line below this paragraph, containing "STRUCTURE_2pmu", creates the "Structure Box" on the page.
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<StructureSection load='2pmu' size='340' side='right'caption='[[2pmu]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pmu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PMU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.779&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
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{{STRUCTURE_2pmu| PDB=2pmu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pmu OCA], [https://pdbe.org/2pmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pmu RCSB], [https://www.ebi.ac.uk/pdbsum/2pmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pmu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7D9B8_MYCTO Q7D9B8_MYCTO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pm/2pmu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pmu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The PhoP-PhoR two-component signaling system from Mycobacterium tuberculosis is essential for the virulence of the tubercle bacillus. The response regulator, PhoP, regulates expression of over 110 genes. In order to elucidate the regulatory mechanism of PhoP, we determined the crystal structure of its DNA-binding domain (PhoPC). PhoPC exhibits a typical fold of the winged helix-turn-helix subfamily of response regulators. The structure starts with a four-stranded antiparallel beta-sheet, followed by a three-helical bundle of alpha-helices, and then a C-terminal beta-hairpin, which together with a short beta-strand between the first and second helices forms a three-stranded antiparallel beta-sheet. Structural elements are packed through a hydrophobic core, with the first helix providing a scaffold for the rest of the domain to pack. The second and third helices and the long, flexible loop between them form the helix-turn-helix motif, with the third helix being the recognition helix. The C-terminal beta-hairpin turn forms the wing motif. The molecular surfaces around the recognition helix and the wing residues show strong positive electrostatic potential, consistent with their roles in DNA binding and nucleotide sequence recognition. The crystal packing of PhoPC gives a hexamer ring, with neighboring molecules interacting in a head-to-tail fashion. This packing interface suggests that PhoPC could bind DNA in a tandem association. However, this mode of DNA binding is likely to be nonspecific because the recognition helix is partially blocked and would be prevented from inserting into the major groove of DNA. Detailed structural analysis and implications with respect to DNA binding are discussed.
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===Crystal structure of the DNA-binding domain of PhoP===
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Structure of the DNA-binding domain of the response regulator PhoP from Mycobacterium tuberculosis.,Wang S, Engohang-Ndong J, Smith I Biochemistry. 2007 Dec 25;46(51):14751-61. Epub 2007 Dec 1. PMID:18052041<ref>PMID:18052041</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2pmu" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18052041}}, adds the Publication Abstract to the page
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*[[PhoP-PhoQ|PhoP-PhoQ]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18052041 is the PubMed ID number.
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*[[Response regulator 3D structure|Response regulator 3D structure]]
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== References ==
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{{ABSTRACT_PUBMED_18052041}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2PMU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PMU OCA].
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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==Reference==
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[[Category: Wang S]]
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Structure of the DNA-binding domain of the response regulator PhoP from Mycobacterium tuberculosis., Wang S, Engohang-Ndong J, Smith I, Biochemistry. 2007 Dec 25;46(51):14751-61. Epub 2007 Dec 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18052041 18052041]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Wang, S.]]
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[[Category: Transcription regulation]]
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[[Category: Winged helix-tun-helx]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:59:03 2008''
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Current revision

Crystal structure of the DNA-binding domain of PhoP

PDB ID 2pmu

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