1mtn

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(New page: 200px<br /><applet load="1mtn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mtn, resolution 2.8&Aring;" /> '''BOVINE ALPHA-CHYMOTRY...)
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[[Image:1mtn.gif|left|200px]]<br /><applet load="1mtn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mtn, resolution 2.8&Aring;" />
 
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'''BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION'''<br />
 
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==Overview==
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==BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION==
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The crystal structure of bovine alpha-chymotrypsin (alpha-CHT) in complex, with the bovine basic pancreatic trypsin inhibitor (BPTI) has been solved, and refined at 2.8 A resolution (R-factor = 0.18). The, proteinase:inhibitor complex forms a compact dimer (two alpha-CHT and two, BPTI molecules), which may be stabilized by surface-bound sulphate ions, in the crystalline state. Each BPTI molecule, at opposite ends, is, contacting both proteinase molecules in the dimer, through the reactive, site loop and through residues next to the inhibitor's C-terminal region., Specific recognition between alpha-CHT and BPTI occurs at the (re)active, site interface according to structural rules inferred from the analysis of, homologous serine proteinase:inhibitor complexes. Lys15, the P1 residue of, BPTI, however, does not occupy the alpha-CHT S1 specificity pocket, being, hydrogen bonded to backbone atoms of the enzyme surface residues Gly216, and Ser217.
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<StructureSection load='1mtn' size='340' side='right'caption='[[1mtn]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mtn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MTN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mtn OCA], [https://pdbe.org/1mtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mtn RCSB], [https://www.ebi.ac.uk/pdbsum/1mtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mtn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CTRA_BOVIN CTRA_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mt/1mtn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mtn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of bovine alpha-chymotrypsin (alpha-CHT) in complex with the bovine basic pancreatic trypsin inhibitor (BPTI) has been solved and refined at 2.8 A resolution (R-factor = 0.18). The proteinase:inhibitor complex forms a compact dimer (two alpha-CHT and two BPTI molecules), which may be stabilized by surface-bound sulphate ions, in the crystalline state. Each BPTI molecule, at opposite ends, is contacting both proteinase molecules in the dimer, through the reactive site loop and through residues next to the inhibitor's C-terminal region. Specific recognition between alpha-CHT and BPTI occurs at the (re)active site interface according to structural rules inferred from the analysis of homologous serine proteinase:inhibitor complexes. Lys15, the P1 residue of BPTI, however, does not occupy the alpha-CHT S1 specificity pocket, being hydrogen bonded to backbone atoms of the enzyme surface residues Gly216 and Ser217.
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==About this Structure==
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Crystal structure of the bovine alpha-chymotrypsin:Kunitz inhibitor complex. An example of multiple protein:protein recognition sites.,Capasso C, Rizzi M, Menegatti E, Ascenzi P, Bolognesi M J Mol Recognit. 1997 Jan-Feb;10(1):26-35. PMID:9179777<ref>PMID:9179777</ref>
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1MTN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MTN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the bovine alpha-chymotrypsin:Kunitz inhibitor complex. An example of multiple protein:protein recognition sites., Capasso C, Rizzi M, Menegatti E, Ascenzi P, Bolognesi M, J Mol Recognit. 1997 Jan-Feb;10(1):26-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9179777 9179777]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 1mtn" style="background-color:#fffaf0;"></div>
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[[Category: Chymotrypsin]]
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[[Category: Protein complex]]
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[[Category: Ascenzi, P.]]
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[[Category: Bolognesi, M.]]
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[[Category: Capasso, C.]]
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[[Category: Menegatti, E.]]
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[[Category: Rizzi, M.]]
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[[Category: SO4]]
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[[Category: complex]]
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[[Category: hydrolase]]
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[[Category: protease inhibitor]]
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[[Category: serine]]
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[[Category: trypsin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:41:19 2007''
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==See Also==
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*[[BPTI 3D structures|BPTI 3D structures]]
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*[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Ascenzi P]]
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[[Category: Bolognesi M]]
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[[Category: Capasso C]]
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[[Category: Menegatti E]]
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[[Category: Rizzi M]]

Current revision

BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION

PDB ID 1mtn

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