1nd9

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{{Seed}}
 
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[[Image:1nd9.png|left|200px]]
 
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==Solution Structure of the N-terminal Subdomain of Translation Initiation Factor IF2==
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The line below this paragraph, containing "STRUCTURE_1nd9", creates the "Structure Box" on the page.
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<StructureSection load='1nd9' size='340' side='right'caption='[[1nd9]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nd9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ND9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ND9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nd9 OCA], [https://pdbe.org/1nd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nd9 RCSB], [https://www.ebi.ac.uk/pdbsum/1nd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nd9 ProSAT]</span></td></tr>
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{{STRUCTURE_1nd9| PDB=1nd9 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IF2_ECOLI IF2_ECOLI] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.[HAMAP-Rule:MF_00100_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nd/1nd9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nd9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 18-kDa Domain I from the N-terminal region of translation initiation factor IF2 from Escherichia coli was expressed, purified, and structurally characterized using multidimensional NMR methods. Residues 2-50 were found to form a compact subdomain containing three short beta-strands and three alpha-helices, folded to form a betaalphaalphabetabetaalpha motif with the three helices packed on the same side of a small twisted beta-sheet. The hydrophobic amino acids in the core of the subdomain are conserved in a wide range of species, indicating that a similarly structured motif is present at the N terminus of IF2 in many of the bacteria. External to the compact 50-amino acid subdomain, residues 51-97 are less conserved and do not appear to form a regular structure, whereas residues 98-157 form a helix containing a repetitive sequence of mostly hydrophilic amino acids. Nitrogen-15 relaxation rate measurements provide evidence that the first 50 residues form a well ordered subdomain, whereas other regions of Domain I are significantly more mobile. The compact subdomain at the N terminus of IF2 shows structural homology to the tRNA anticodon stem contact fold domains of the methionyl-tRNA and glutaminyl-tRNA synthetases, and a similar fold is also found in the B5 domain of the phenylalanine-tRNA synthetase. The results of the present work will provide guidance for the design of future experiments directed toward understanding the functional roles of this widely conserved structural domain within IF2.
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===Solution Structure of the N-terminal Subdomain of Translation Initiation Factor IF2===
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A conserved structural motif at the N terminus of bacterial translation initiation factor IF2.,Laursen BS, Mortensen KK, Sperling-Petersen HU, Hoffman DW J Biol Chem. 2003 May 2;278(18):16320-8. Epub 2003 Feb 24. PMID:12600987<ref>PMID:12600987</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12600987}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1nd9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12600987 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12600987}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1ND9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ND9 OCA].
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==Reference==
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A conserved structural motif at the N terminus of bacterial translation initiation factor IF2., Laursen BS, Mortensen KK, Sperling-Petersen HU, Hoffman DW, J Biol Chem. 2003 May 2;278(18):16320-8. Epub 2003 Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12600987 12600987]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hoffman, D W.]]
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[[Category: Hoffman DW]]
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[[Category: Laursen, B S.]]
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[[Category: Laursen BS]]
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[[Category: Mortensen, K K.]]
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[[Category: Mortensen KK]]
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[[Category: Sperling-Petersen, H U.]]
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[[Category: Sperling-Petersen HU]]
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[[Category: If2]]
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[[Category: Initiation factor]]
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[[Category: Translation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:19:19 2008''
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Current revision

Solution Structure of the N-terminal Subdomain of Translation Initiation Factor IF2

PDB ID 1nd9

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